Type

Journal Article

Authors

M A Crowe
P. Lonergan
J. F. Roche
P. Ó'Gaora
J. P. Mehta
P. Duffy
G. B. Duffy
M E Beltman
N Forde

Subjects

Biochemistry

Topics
animals progesterone dinoprost female down regulation embryo transfer receptors progesterone cattle pregnancy estrous cycle genetics principal component analysis metabolism gene expression profiling blood endometrium embryonic development time factors drug effects gene expression osmolar concentration pharmacology embryo mammalian

Changes in the endometrial transcriptome during the bovine estrous cycle: effect of low circulating progesterone and consequences for conceptus elongation. (2010)

Abstract In cattle, elevated concentrations of circulating progesterone (P4) in the immediate postconception period are associated with advanced conceptus development, while low P4 is implicated as a causative factor in low pregnancy rates observed in dairy cows. This study aimed to: 1) describe the transcriptional changes that occur in the bovine endometrium during the estrous cycle, 2) determine how elevated P4 affects these changes, 3) identify if low P4 alters the expression of these genes, and 4) assess the impact that low P4 has on conceptus development. Relatively few differences occurred in endometrial gene expression during the early luteal phase of the estrous cycle (Day 5 vs. 7), but comparison of endometria from more distant stages of the luteal phase (Day 7 vs. 13) revealed large transcriptional changes, which were significantly altered by exogenous supplementation of P4. Induction of low circulating P4 altered the normal temporal changes in gene expression, and these changes were coordinate with a delay in the down-regulation of the PGR from the LE and GE. Altered endometrial gene expression induced by low P4 was associated with a reduced capacity of the uterus to support conceptus development after embryo transfer on Day 7. In conclusion, the present study provides clear evidence that the temporal changes in the transcriptome of the endometrium of cyclic heifers are sensitive to circulating P4 concentrations in the first few days after estrus. Under low P4 conditions, a suboptimal uterine environment with reduced ability to support conceptus elongation is observed.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M A Crowe, P. Lonergan, J. F. Roche, P. Ó'Gaora, J. P. Mehta, P. Duffy, G. B. Duffy, M E Beltman, N Forde

Experts in our system

1
M A Crowe
University College Dublin
Total Publications: 101
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
J. F. Roche
University College Dublin
Total Publications: 111
 
4
Jai Prakash Mehta
University College Dublin
Total Publications: 29
 
5
P. Duffy
University College Dublin
Total Publications: 42
 
6
G Duffy
Teagasc
Total Publications: 92
 
7
Marijke Eileen Beltman
University College Dublin
Total Publications: 24
 
8
N Forde
University College Dublin
Total Publications: 53