Type

Journal Article

Authors

Pauline M Rudd
Michael J Duffy
Denis Evoy
Enda W McDermott
Jodie L Abrahams
Umi M Abd Hamid
Radka Saldova
Aisling Pierce

Subjects

Medicine & Nursing

Topics
chromatography high pressure liquid breast neoplasms prognosis pathology secondary carcinoma ductal breast neoplasm staging lymphatic metastasis carcinoma lobular blood adenocarcinoma mucinous metabolism humans middle aged lymph nodes polysaccharides axilla sialyl lewis x antigen female antigens cd15

Levels of specific glycans significantly distinguish lymph node-positive from lymph node-negative breast cancer patients. (2010)

Abstract One of the most urgent requirements in breast cancer is the development of a blood-based test for early detection and prognosis. Previously published results found a significant difference between specific glycan levels in patients with advanced breast cancer and healthy controls. The aim of this investigation was to address a more clinically relevant problem, i.e., whether the measurement of specific glycans could identify women with aggressive disease at an early stage. In order to reduce potential bias in this study, blood samples from patients were collected, stored and analyzed in a similar manner. Agalactosyl biantennary glycans (FA2) and glycans containing the sialyl Lewis x epitope (A3F1G1 and A2F1G1) were measured using high throughput normal-phase high-performance liquid chromatography in combination with exoglycosidase digestions in sera from 52 patients with early breast cancer (21 with lymph node-negative and 20 with lymph node-positive disease) and 134 women with benign breast disease. The combined levels of the glycans were significantly higher in patients with lymph node metastases compared to women without these metastases. Lymph node status is the single most important determinant of survival in early stage breast cancer. As high levels of these glycans were associated with nodal metastases, their measurement may provide a new non-invasive approach to determining prognosis in women with newly diagnosed breast cancer.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Pauline M Rudd, Michael J Duffy, Denis Evoy, Enda W McDermott, Jodie L Abrahams, Umi M Abd Hamid, Radka Saldova, Aisling Pierce

Experts in our system

1
Pauline M Rudd
University College Dublin
Total Publications: 45
 
2
Michael J Duffy
University College Dublin
 
3
E W McDermott
University College Dublin
Total Publications: 49
 
4
Radka Saldova
Dublin City University