Type

Journal Article

Authors

Emmeline W Hill
Lisa M Katz
Barbara A Murphy
David E. MacHugh
Amanda Lohan
Brendan J Loftus
Rita G Fonseca
Alexander C O Evans
John A Browne
Paul A. McGettigan
and 1 others

Subjects

Biochemistry

Topics
female animals genetics gene expression profiling metabolism mice male reverse transcriptase polymerase chain reaction muscle skeletal physical conditioning animal humans time factors gene library physiology horses reproducibility of results

Characterization of the equine skeletal muscle transcriptome identifies novel functional responses to exercise training. (2010)

Abstract Digital gene expression profiling was used to characterize the assembly of genes expressed in equine skeletal muscle and to identify the subset of genes that were differentially expressed following a ten-month period of exercise training. The study cohort comprised seven Thoroughbred racehorses from a single training yard. Skeletal muscle biopsies were collected at rest from the gluteus medius at two time points: T(1) - untrained, (9 +/- 0.5 months old) and T(2) - trained (20 +/- 0.7 months old). The most abundant mRNA transcripts in the muscle transcriptome were those involved in muscle contraction, aerobic respiration and mitochondrial function. A previously unreported over-representation of genes related to RNA processing, the stress response and proteolysis was observed. Following training 92 tags were differentially expressed of which 74 were annotated. Sixteen genes showed increased expression, including the mitochondrial genes ACADVL, MRPS21 and SLC25A29 encoded by the nuclear genome. Among the 58 genes with decreased expression, MSTN, a negative regulator of muscle growth, had the greatest decrease.Functional analysis of all expressed genes using FatiScan revealed an asymmetric distribution of 482 Gene Ontology (GO) groups and 18 KEGG pathways. Functional groups displaying highly significant (P < 0.0001) increased expression included mitochondrion, oxidative phosphorylation and fatty acid metabolism while functional groups with decreased expression were mainly associated with structural genes and included the sarcoplasm, laminin complex and cytoskeleton. Exercise training in Thoroughbred racehorses results in coordinate changes in the gene expression of functional groups of genes related to metabolism, oxidative phosphorylation and muscle structure.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Emmeline W Hill, Lisa M Katz, Barbara A Murphy, David E. MacHugh, Amanda Lohan, Brendan J Loftus, Rita G Fonseca, Alexander C O Evans, John A Browne, Paul A. McGettigan and 1 others

Experts in our system

1
Emmeline W Hill
University College Dublin
Total Publications: 31
 
2
Lisa M Katz
University College Dublin
Total Publications: 22
 
3
David E. MacHugh
University College Dublin
Total Publications: 82
 
4
Brendan Loftus
University College Dublin
Total Publications: 21
 
5
Alexander C O Evans
University College Dublin
Total Publications: 14
 
6
John A Browne
University College Dublin
Total Publications: 73
 
7
Paul A. McGettigan
University College Dublin
Total Publications: 46