Type

Journal Article

Authors

J E Nally
B E Markey
Julian P Whitelegge
H F Basset
D Sammin
C Brady
A Proctor
M McElroy
S Worrall
E J Gutierrez
and 4 others

Subjects

Microbiology

Topics
sheep bacterial proteins sheep diseases antibodies bacterial pregnancy complications infectious chlamydophila antigens bacterial animals immunology pregnancy disease models animal female electrophoresis gel two dimensional blotting western aborted fetus microbiology chlamydophila infections electrophoresis polyacrylamide gel blood veterinary mass spectrometry

Identification of immunologically relevant proteins of Chlamydophila abortus using sera from experimentally infected pregnant ewes. (2010)

Abstract Chlamydophila abortus is an intracellular pathogen and the etiological agent of enzootic abortion of ewes (EAE). C. abortus has a biphasic development cycle; extracellular infectious elementary bodies (EB) attach and penetrate host cells, where they give rise to intracellular, metabolically active reticulate bodies (RB). RB divide by binary fission and subsequently mature to EB, which, on rupture of infected cells, are released to infect new host cells. Pregnant ewes were challenged with 2 x 10(6) inclusion forming units (IFU) of C. abortus cultured in yolk sac (comprising both EB and RB). Serum samples were collected at 0, 7, 14, 21, 27, 30, 35, 40, and 43 days postinfection (dpi) and used to identify antigens of C. abortus expressed during disease. Additionally, sera from fetal lambs were collected at 30, 35, 40, and 43 dpi. All serum samples collected from experimentally infected pregnant ewes reacted specifically with several antigens of EB as determined by one-dimensional (1-D) and 2-D gel electrophoresis; reactive antigens identified by mass spectrometry included the major outer membrane protein (MOMP), polymorphic outer membrane protein (POMP), and macrophage infectivity potentiator (MIP) lipoprotein.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

J E Nally, B E Markey, Julian P Whitelegge, H F Basset, D Sammin, C Brady, A Proctor, M McElroy, S Worrall, E J Gutierrez and 4 others

Experts in our system

1
Jarlath E Nally
University College Dublin
Total Publications: 28
 
2
D Sammin
University College Dublin
Total Publications: 14
 
3
C Brady
University College Cork
Total Publications: 7
 
4
Máire C McElroy
Teagasc
Total Publications: 28