Type

Journal Article

Authors

Leonie S Young
Arnold D Hill
E McDermott
Thomas B Crotty
Fiona Bane
Eddie Myers
Marie McIlroy
Dara O Kavanagh

Subjects

Microbiology

Topics
receptor protein tyrosine kinases adult receptor erbb 2 humans adenocarcinoma follicular aged 80 and over tumor cells cultured adolescent estrogen receptor alpha female case control studies carcinoma metabolism survival analysis male young adult middle aged co repressor proteins mortality aged physiology transcription factors thyroid neoplasms child etiology trans activators erbb2 protein human child preschool

The role of oestrogen receptor {alpha} in human thyroid cancer: contributions from coregulatory proteins and the tyrosine kinase receptor HER2. (2009)

Abstract Epidemiological, clinical, and molecular studies suggest a role for oestrogen in thyroid cancer. How oestrogen mediates its effects and the consequence of it on clinical outcome has not been fully elucidated. The participation of coregulatory proteins in modulating oestrogen receptor (ER) function and input of crosstalk with the tyrosine kinase receptor HER2 was investigated. Oestrogen induced cell proliferation in the follicular thyroid cancer (FTC)-133 cells, but not in the anaplastic 8305C cell line. Knockdown of the coactivator steroid receptor coactivator (SRC)-1 inhibited FTC-133 basal, but not oestrogen induced, cell proliferation. Oestrogen also increased protein expression of SRC-1 and the ER target gene cyclin D1 in the FTC-133 cell line. ERalpha, ERbeta, the coregulatory proteins SRC-1 and nuclear corepressor (NCoR), and the tyrosine kinase receptor HER2 were localised by immunohistochemistry and immnofluorescence in paraffin-embedded tissue from thyroid tumour patients (n=111). ERalpha was colocalised with both SRC-1 and NCoR to the nuclei of the tumour epithelial cells. Expression of ERalpha and NCoR was found predominantly in non-anaplastic tumours and was significantly associated with well-differentiated tumours and reduced incidence of disease recurrence. In non-anaplastic tumours, HER2 was significantly associated with SRC-1, and these proteins were associated with poorly differentiated tumours, capsular invasion and disease recurrence. Totally, 87% of anaplastic tumours were positive for SRC-1. Kaplan-Meier estimates of disease-free survival indicated that in thyroid cancer, SRC-1 strongly correlates with reduced disease-free survival (P<0.001), whereas NCoR predicted increased survival (P<0.001). These data suggest opposing roles for the coregulators SRC-1 and NCoR in thyroid tumour progression.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Leonie S Young, Arnold D Hill, E McDermott, Thomas B Crotty, Fiona Bane, Eddie Myers, Marie McIlroy, Dara O Kavanagh

Experts in our system

1
Leonie S Young
Royal College of Surgeons in Ireland
Total Publications: 42
 
2
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
 
3
E W McDermott
University College Dublin
Total Publications: 49
 
4
Marie McIlroy
Royal College of Surgeons in Ireland