Type

Journal Article

Authors

William M Gallagher
Stephen R Pennington
Michael J Duffy
Susan McDonnell
Isabelle de Gardelle
Sophie Dijon
Karen A Power
Thomas Y K Lau

Subjects

Microbiology

Topics
humans chemistry female electrophoresis gel two dimensional disease progression blood proteins genetics tumor markers biological neoplasm proteins gene expression regulation neoplastic computer simulation biosynthesis kaplan meier estimate blood cell line tumor breast neoplasms reproducibility of results culture media conditioned models biological mass spectrometry pathology

Prioritization of candidate protein biomarkers from an in vitro model system of breast tumor progression toward clinical verification. (2009)

Abstract The use of in vitro cell culture model systems has revealed many potential mediators and candidate biomarkers of various disease phenotypes. To be of clinical utility, the expression of these candidates must be assessed in patient samples such as tissue, urine or blood. However, typical "omic" experiments may produce candidates in such large numbers that it is usually impossible to test all of these in clinical samples. Here, we present a proteomic approach to discover and prioritize candidate biomarkers that are more likely to be found in serum. Using a combination of experimental and in silico approaches, we have demonstrated this approach using an isogenic cell culture model of breast cancer invasion. Differential proteomics (2D-DIGE) was used to discover a number of candidate biomarkers and a subset of these were identified as "extracellular". We tested the validity of this approach by screening serum from breast cancer patients for these candidates and then verified the presence of several of these "extracellular" proteins. This approach provides a pragmatic approach to prioritizing candidates that may be most suitable for downstream assays such as multiple reaction monitoring.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

William M Gallagher, Stephen R Pennington, Michael J Duffy, Susan McDonnell, Isabelle de Gardelle, Sophie Dijon, Karen A Power, Thomas Y K Lau

Experts in our system

1
William M Gallagher
University College Dublin
Total Publications: 148
 
2
Stephen R Pennington
University College Dublin
 
3
Michael J Duffy
University College Dublin
 
4
Susan McDonnell
University College Dublin
Total Publications: 26
 
5
Thomas Y K Lau
University College Dublin
Total Publications: 7