Type

Journal Article

Authors

Kevin E O'Connor
Jasmina Nikodinovic-Runic
Aisling R Hume

Subjects

Biochemistry

Topics
kinetics coenzyme a ligases enzymology caprylates bacterial proteins substrate specificity 2 bromooctanoic acid genetics chemistry metabolism metabolic networks and pathways pseudomonas putida acrylates gene dosage culture media acrylic acid polyhydroxyalkanoates growth development fatty acids gene deletion enzyme inhibitors models biological pharmacology

FadD from Pseudomonas putida CA-3 is a true long-chain fatty acyl coenzyme A synthetase that activates phenylalkanoic and alkanoic acids. (2009)

Abstract A fatty acyl coenzyme A synthetase (FadD) from Pseudomonas putida CA-3 is capable of activating a wide range of phenylalkanoic and alkanoic acids. It exhibits the highest rates of reaction and catalytic efficiency with long-chain aromatic and aliphatic substrates. FadD exhibits higher k(cat) and K(m) values for aromatic substrates than for the aliphatic equivalents (e.g., 15-phenylpentadecanoic acid versus pentadecanoic acid). FadD is inhibited noncompetitively by both acrylic acid and 2-bromooctanoic acid. The deletion of the fadD gene from P. putida CA-3 resulted in no detectable growth or polyhydroxyalkanoate (PHA) accumulation with 10-phenyldecanoic acid, decanoic acid, and longer-chain substrates. The results suggest that FadD is solely responsible for the activation of long-chain phenylalkanoic and alkanoic acids. While the CA-3DeltafadD mutant could grow on medium-chain substrates, a decrease in growth yield and PHA accumulation was observed. The PHA accumulated by CA-3DeltafadD contained a greater proportion of short-chain monomers than did wild-type PHA. Growth of CA-3DeltafadD was unaffected, but PHA accumulation decreased modestly with shorter-chain substrates. The complemented mutant regained 70% to 90% of the growth and PHA-accumulating ability of the wild-type strain depending on the substrate. The expression of an extra copy of fadD in P. putida CA-3 resulted in increased levels of PHA accumulation (up to 1.6-fold) and an increase in the incorporation of longer-monomer units into the PHA polymer.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Kevin E O'Connor, Jasmina Nikodinovic-Runic, Aisling R Hume

Experts in our system

1
Kevin E O'Connor
University College Dublin
Total Publications: 52
 
2
Jasmina Nikodinovic-Runic
University College Dublin
Total Publications: 19