Type

Journal Article

Authors

Trudee Fair
Patrick Lonergan
Ines Maria Aparicio Donoso
Grace M O'Gorman
Shirley A Ellis
Julie Doyle

Subjects

Biochemistry

Topics
minor histocompatibility antigens immunology immunochemistry morula cattle organ culture techniques histocompatibility antigens class i animals oocytes genetics gene expression profiling metabolism embryonic development fertilization in vitro blastocyst gene expression regulation developmental biological markers zygote

Classical and non-classical Major Histocompatibility Complex class I gene expression in in vitro derived bovine embryos. (2008)

Abstract The role of the Major Histocompatibility Complex class I (MHC-I) genes in human and mouse preimplantation embryo development has received considerable attention. In contrast, information concerning the role of these genes in bovine embryo development is limited. The objective of the current study was to characterize the expression pattern of MHC-I genes during preimplantation embryo development in cattle. To this end, bovine oocytes were harvested from slaughterhouse ovaries, matured, fertilized and cultured in vitro. Samples were collected at immature and mature oocyte, presumptive zygote, 2-4-cell embryo, 8-16-cell embryo, morula, blastocyst and hatched blastocyst stages of development. MHC-I expression was detected using quantitative real-time-PCR, cDNA sequencing, whole mount immunocytochemistry and Western blotting. We report classical and non-classical MHC-I mRNA expression in bovine oocytes and developing embryos. Furthermore, we report that the pattern of MHC-I mRNA expression across development was gene- and stage-specific.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Trudee Fair, Patrick Lonergan, Ines Maria Aparicio Donoso, Grace M O'Gorman, Shirley A Ellis, Julie Doyle

Experts in our system

1
T Fair
University College Dublin
Total Publications: 66
 
2
P. Lonergan
University College Dublin
Total Publications: 190