Type

Journal Article

Authors

Kevin E O'Connor
Werner Blau
Ramesh Babu
Leona B Martin
Jasmina Nikodinovic-Runic

Subjects

Biochemistry

Topics
biomass monophenol monooxygenase genetics radiation effects microbial viability toxicity ultraviolet rays pseudomonas putida biosynthesis hydrogen peroxide tyrosine growth development melanins homogentisate 1 2 dioxygenase transcription factors ferulic acid metabolism coumaric acids dna transposable elements mutagenesis insertional

Characterization of melanin-overproducing transposon mutants of Pseudomonas putida F6. (2009)

Abstract Two melanin-overproducing Pseudomonas putida F6 mutants were generated using transposon (Tn5) mutagenesis. Mutants were disrupted in a transcriptional regulator (TR) and a homogentisate 1,2-dioxygenase (HDO) gene. Colonies of mutant F6-TR overproduced a black pigment on solid medium. The same mutant (F6-TR) had a 3.7-fold higher tyrosinase activity compared with the wild-type strain when induced with ferulic acid. However in tyrosine uptake assays whole cells of the mutant strain F6-TR consumed eight times less tyrosine compared with the wild-type strain. Mutant F6-HDO produced a diffusible red pigment into the growth medium. Pigment production by mutant F6-HDO is sixfold higher than the wild-type strain. The biomass yield of mutant F6-HDO grown on tyrosine as the sole source of carbon and energy was 1.2-fold lower than the wild-type strain. While the growth of the wild-type strain was completely inhibited by 5 min of exposure to UV light (254 nm) both mutant strains showed survival rates >30%. Mutant F6-HDO was able to tolerate higher concentrations of hydrogen peroxide (H(2)O(2)) exhibiting 1.5 times smaller zones of inhibition at 10 mM H(2)O(2) compared with mutant F6-TR and the wild-type strain. The pigments produced by all strains were purified and confirmed to be melanins.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Kevin E O'Connor, Werner Blau, Ramesh Babu, Leona B Martin, Jasmina Nikodinovic-Runic

Experts in our system

1
Kevin E O'Connor
University College Dublin
Total Publications: 52
 
2
Werner Blau
Trinity College Dublin
Total Publications: 109
 
3
Ramesh P Babu
University College Dublin
Total Publications: 11
 
4
Jasmina Nikodinovic-Runic
University College Dublin
Total Publications: 19