Journal Article


Kevin E O'Connor
Jasmina Nikodinovic-Runic
Miriam Goff



pseudomonas putida carbon biomass bacterial proteins hot temperature nitrogen peptide hydrolases genetics lipopolysaccharides transaminases metabolism growth development mutagenesis insertional polyhydroxyalkanoates phenylalanine aminotransferase dna transposable elements

Characterization of temperature-sensitive and lipopolysaccharide overproducing transposon mutants of Pseudomonas putida CA-3 affected in PHA accumulation. (2009)

Abstract A library of 20 000 transposon (Tn5) mutants of the gram-negative bacterium Pseudomonas putida CA-3 was generated and screened for adverse affects in polyhydroxyalkanoates (PHA) accumulation. Two mutants of interest were characterized phenotypically. CA-3-126, a mutant disrupted in a stress-related protein Clp protease subunit ClpA, demonstrated greater decreases in PHA accumulation compared with the wild type at reduced and elevated temperatures under PHA-accumulating growth conditions. CA-3-M, which is affected in the aminotransferase class I enzyme, accumulated reduced levels of PHA relative to the wild type and had lower growth yields on all carbon sources tested. Mutant CA-3-M produced up to 10-fold higher levels of lipopolysaccharide relative to the wild type and exhibited 1.2-fold lower aminotransferase activity with phenylalanine as a substrate compared with the wild-type strain. The composition of the lipopolysaccharide produced by the mutant differed from that produced by the wild-type strain. Growth and PHA accumulation by CA-3-M was the same as the wild type when the nitrogen concentration in the medium was increased to 265 mg N L(-1).
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Full list of authors on original publication

Kevin E O'Connor, Jasmina Nikodinovic-Runic, Miriam Goff

Experts in our system

Kevin E O'Connor
University College Dublin
Total Publications: 52
Jasmina Nikodinovic-Runic
University College Dublin
Total Publications: 19