Type

Journal Article

Authors

Eamonn Gormley
Mark Chambers
R Glyn Hewinson
Mahavir Singh
Javan Esfandiari
Rena Greenwald
Konstantin Lyashchenko
Eamon Costello
Leigh Corner
Sandrine Lesellier

Subjects

Microbiology

Topics
microbiology immunoglobulin g cattle pathology tuberculosis bovine lung interferon gamma 1 39 disease reservoirs blood mycobacterium bovis antibodies bacterial leukocytes mononuclear colony count microbial secretion interferon gamma peptide fragments animals mustelidae severity of illness index bcg vaccine immunology prevention control

Immunological responses and protective immunity in BCG vaccinated badgers following endobronchial infection with Mycobacterium bovis. (2008)

Abstract European badgers (Meles meles) are a reservoir host of Mycobacterium bovis and are implicated in the transmission of tuberculosis to cattle in Ireland and Great Britain. The development of a vaccine for use in badgers is considered a key element of any campaign to eradicate the disease in livestock in both countries. In this study we have vaccinated groups of badgers with approximately 5 x 10(5)cfu of the BCG vaccine delivered via two alternative routes, subcutaneous and mucosal (intranasal/conjunctival). Following experimental endobronchial infection with approximately 10(4)cfu of M. bovis, all badgers were euthanised at 12 weeks post-infection. At post-mortem examination both vaccinated groups had significantly reduced severity of disease compared with the non-vaccinated controls. The analysis of immune responses throughout the study showed that vaccination with BCG did not generate any detectable immunological responses as measured by IFN-gamma production in antigen-stimulated peripheral blood mononuclear cells (PBMC) and IgG serological responses. However, the levels of the responses increased following M. bovis infection, and the kinetic profiles corresponded to the severity of lesions recorded post-mortem. Significant differences were observed in the timing of development of the immune responses between vaccinates and controls. The results suggest that the immunological responses are associated with the levels of protective immunity and could be used as markers to monitor control of disease in badgers following vaccination.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Eamonn Gormley, Mark Chambers, R Glyn Hewinson, Mahavir Singh, Javan Esfandiari, Rena Greenwald, Konstantin Lyashchenko, Eamon Costello, Leigh Corner, Sandrine Lesellier

Experts in our system

1
E Gormley
University College Dublin
Total Publications: 68
 
2
Mark Chambers
University College Dublin
Total Publications: 9
 
3
Eamon Costello
Dublin City University
Total Publications: 94
 
4
L A L Corner
University College Dublin
Total Publications: 34
 
5
Sandrine Lesellier
University College Dublin
Total Publications: 14