Journal Article


Eamonn Gormley
Damien O'Meara
Sandrine Lesellier
Eamon Costello
Leigh A L Corner



female prevention control administration oral mycobacterium bovis cattle immunology adjuvants immunologic animals pathogenicity pathology tuberculosis bovine lung male veterinary administration dosage disease reservoirs great britain mucous membrane colony count microbial severity of illness index ireland tuberculosis mustelidae bcg vaccine

Vaccination of European badgers (Meles meles) with BCG by the subcutaneous and mucosal routes induces protective immunity against endobronchial challenge with Mycobacterium bovis. (2007)

Abstract Mycobacterium bovis is endemic in badger (Meles meles) populations of Ireland and the United Kingdom and infected badgers are a potential source of infection for cattle. In domestic livestock tuberculosis causes economic losses from lost production and the costs associated with eradication programmes, and in addition there is a risk of zoonotic infection. Whereas culling is currently used to control tuberculous badger populations in Ireland, vaccination, if it were available, would be preferred. A study was undertaken to examine the protective responses of badgers vaccinated either by the subcutaneous or mucosal (intranasal and conjunctival) routes with bacille Calmette-Guérin (BCG), when challenged with M. bovis by the endobronchial route. Three groups of badgers were used. The first group (n=4) was vaccinated with approximately 5 x 10(5) colony forming units (cfu) of BCG by subcutaneous injection. In the second group (n=5) badgers were vaccinated via the mucosal route by instilling 1.0 x 10(5)cfu into each conjunctival sac and spraying 1.0 x 10(5)cfu into each nostril (final vaccine dose of 4 x 10(5)cfu). The control (n=5) badgers served as a non-vaccinated group. Twelve weeks post-vaccination all badgers in the three groups were challenged with approximately 10(4)cfu of M. bovis by endobronchial inoculation. At 12 weeks post-infection all badgers were examined post-mortem to assess the pathological and bacteriological responses to challenge. Gross and histological lesions of tuberculosis were seen in all challenged badgers and M. bovis was recovered from all challenged badgers. However, across six of the eight parameters used to measure disease severity, the infection in the vaccinated badgers was significantly less severe than in the control group. The BCG vaccine induced a significant protective effect in the badgers and the protective immunity was generated by subcutaneous and mucosal vaccination.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Eamonn Gormley, Damien O'Meara, Sandrine Lesellier, Eamon Costello, Leigh A L Corner

Experts in our system

E Gormley
University College Dublin
Total Publications: 68
Sandrine Lesellier
University College Dublin
Total Publications: 14
Eamon Costello
Dublin City University
Total Publications: 94
L A L Corner
University College Dublin
Total Publications: 34