Type

Journal Article

Authors

William M Gallagher
Stephen M Hewitt
Michael J Duffy
Goran Landberg
Robert C Millikan
Karin Jirstrom
Desmond G Higgins
Aedín C Culhane
Ailís Fagan
Darran P O'Connor
and 4 others

Subjects

Microbiology

Topics
cytoplasm female inhibitor of apoptosis proteins tissue array analysis metabolism diagnosis birc5 protein human prognosis cell nucleus tumor markers biological neoplasm proteins regression analysis microtubule associated proteins middle aged adult cell line tumor breast neoplasms humans mortality aged aged 80 and over

Altered cytoplasmic-to-nuclear ratio of survivin is a prognostic indicator in breast cancer. (2008)

Abstract Survivin (BIRC5) is a promising tumor biomarker. Conflicting data exist on its prognostic effect in breast cancer. These data may at least be partly due to the manual interpretation of immunohistochemical staining, especially as survivin can be located in both the nucleus and cytoplasm. Quantitative determination of survivin expression using image analysis offers the opportunity to develop alternative scoring models for survivin immunohistochemistry. Here, we present such a model. A breast cancer tissue microarray containing 102 tumors was stained with an anti-survivin antibody. Whole-slide scanning was used to capture high-resolution images. These images were analyzed using automated algorithms to quantify the staining. Increased nuclear, but not cytoplasmic, survivin was associated with a reduced overall survival (OS; P = 0.038) and disease-specific survival (P = 0.0015). A high cytoplasmic-to-nuclear ratio (CNR) of survivin was associated with improved OS (P = 0.005) and disease-specific survival (P = 0.05). Multivariate analysis revealed that the survivin CNR was an independent predictor of OS (hazard ratio, 0.09; 95% confidence interval, 0.01-0.76; P = 0.027). A survivin CNR of >5 correlated positively with estrogen receptor (P = 0.019) and progesterone receptor (P = 0.033) levels, whereas it was negatively associated with Ki-67 expression (P = 0.04), p53 status (P = 0.005), and c-myc amplification (P = 0.016). Different prognostic information is supplied by nuclear and cytoplasmic survivin in breast cancer. Nuclear survivin is a poor prognostic marker in breast cancer. Moreover, CNR of survivin, as determined by image analysis, is an independent prognostic factor.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

William M Gallagher, Stephen M Hewitt, Michael J Duffy, Goran Landberg, Robert C Millikan, Karin Jirstrom, Desmond G Higgins, Aedín C Culhane, Ailís Fagan, Darran P O'Connor and 4 others

Experts in our system

1
William M Gallagher
University College Dublin
Total Publications: 148
 
2
Michael J Duffy
University College Dublin
 
3
Karin Jirstrom
University College Dublin
 
4
Desmond G Higgins
University College Dublin
Total Publications: 76
 
5
Ailís Fagan
Royal College of Surgeons in Ireland
Total Publications: 13
 
6
Darran P O'Connor
Royal College of Surgeons in Ireland
Total Publications: 43