Type

Journal Article

Authors

A.C.O. Evans
J J Ireland
P M Coussens
G W Smith
P. Lonergan
S Park
P J Baker
A E Zielak
M J Canty
M Mihm
and 1 others

Subjects

Biochemistry

Topics
animals enzymology betaglycan rna messenger gene expression profiling receptors lh cells cultured ephrins cytology gene expression regulation carrier proteins progesterone female rna small interfering cattle genetics granulosa cells metabolism signal transduction estradiol calcium calmodulin dependent protein kinase type proteoglycans receptors fsh theca cells receptors transforming growth factor beta

Differential expression of signal transduction factors in ovarian follicle development: a functional role for betaglycan and FIBP in granulosa cells in cattle. (2008)

Abstract Ovarian follicles develop in groups yet individual follicles follow different growth trajectories. This growth and development are regulated by endocrine and locally produced growth factors that use a myriad of receptors and signal transduction pathways to exert their effects on theca and granulosa cells. We hypothesize that differential growth may be due to differences in hormonal responsiveness that is partially mediated by differences in expression of genes involved in signal transduction. We used the bovine dominant follicle model, microarrays, quantitative real-time PCR and RNA interference to examine this. We identified 83 genes coding for signal transduction molecules and validated a subset of them associated with different stages of the follicle wave. We suggest important roles for CAM kinase-1 and EphA4 in theca cells and BCAR1 in granulosa cells for the development of dominant follicles and for betaglycan and FIBP in granulosa cells of regressing subordinate follicles. Inhibition of genes for betaglycan and FIBP in granulosa cells in vitro suggests that they inhibit estradiol production in regressing subordinate follicles.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

A.C.O. Evans, J J Ireland, P M Coussens, G W Smith, P. Lonergan, S Park, P J Baker, A E Zielak, M J Canty, M Mihm and 1 others

Experts in our system

1
A.C.O. Evans
University College Dublin
Total Publications: 92
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
Stephen D. E. Park
University College Dublin
Total Publications: 35