Type

Journal Article

Authors

Eamonn Gormley
Mark Chambers
R Glyn Hewinson
Mahavir Singh
Javan Esfandiari
Rena Greenwald
Konstantin Lyashchenko
Paddy Sleeman
Eamon Costello
Leigh Corner
and 1 others

Subjects

Microbiology

Topics
mustelidae female mycobacterium bovis microbiology immunology antibodies bacterial tuberculin animals antigens bacterial lymphocyte activation blood male immunoglobulin g

Antigen specific immunological responses of badgers (Meles meles) experimentally infected with Mycobacterium bovis. (2007)

Abstract European badgers (Meles meles) are considered to be an important reservoir of infection for Mycobacterium bovis and are implicated in the transmission of tuberculosis to cattle in Ireland and Great Britain. Accurate tests are required for tuberculosis surveillance in badger populations and to provide a basis for the development of strategies, including vaccination, to reduce the incidence of the infection. In this study, we have developed an endobronchial M. bovis infection model in badgers in which we measured cell-mediated immune and serological responses for up to 24 weeks post-infection. Groups of badgers were subjected to necropsy at 6-week intervals and the gross lesion severity status compared with immune responses measured in blood samples taken throughout the course of the study. The panel of antigens included bovine and avian tuberculins (PPD) as well as single antigens, ESAT-6, CFP-10, MPB70, Rv3019c, Rv3873, Rv3878 and Rv3879, all known to be recognised by the immune system in other animal models of tuberculosis infection. Our results demonstrated that M. bovis infected badgers responded to specific antigens as early as 6 weeks post-infection, consistent with the presence of visible lesions. The data also revealed unique patterns of antigen recognition with high levels of PBMC proliferation in the presence of CFP-10 but low proliferation levels with ESAT-6. Using a multi-antigen print immunoassay (MAPIA), we were able to confirm that MPB83 is the dominant antigen recognised by serum antibodies in infected badgers.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Eamonn Gormley, Mark Chambers, R Glyn Hewinson, Mahavir Singh, Javan Esfandiari, Rena Greenwald, Konstantin Lyashchenko, Paddy Sleeman, Eamon Costello, Leigh Corner and 1 others

Experts in our system

1
E Gormley
University College Dublin
Total Publications: 68
 
2
Mark Chambers
University College Dublin
Total Publications: 9
 
3
Eamon Costello
Dublin City University
Total Publications: 94
 
4
L A L Corner
University College Dublin
Total Publications: 34