Type

Journal Article

Authors

Keith J. Murphy
Ciaran M Regan
Desmond G Higgins
Paul N Moynagh
John J O'Connor
Lisa Conboy
Mark Pickering
Graham K. Sheridan
Paul A. McGettigan
Niamh C O'Sullivan

Subjects

Biochemistry

Topics
cytology reverse transcriptase polymerase chain reaction models biological avoidance learning reaction time gene expression down regulation gene expression regulation rats wistar h 3 behavior animal metabolism up regulation neurons microarray analysis blotting western male dentate gyrus transcription factors passive avoidance h 2 rats physiology time factors computational biology animals

Temporal change in gene expression in the rat dentate gyrus following passive avoidance learning. (2007)

Abstract A learning event initiates a cascade of altered gene expression leading to synaptic remodelling within the hippocampal dentate gyrus, a structure vital to memory formation. To illuminate this transcriptional program of synaptic plasticity we used microarrays to quantify mRNA from the rat dentate gyrus at increasing times following passive avoidance learning. Approximately, 500 known genes were transcriptionally regulated across the 24 h post-training period. The 0-2 h period saw up-regulation of genes involved in transcription while genes with a role in synaptic/cytoskeletal structure increased 0-6 h, consistent with structural rearrangements known to occur at these times. The most striking feature was the profound down-regulation, across all functional groups, 12 h post-training. Bioinformatics analysis identified the likely transcription factors controlling gene expression in each post-training period. The role of NF kappa B, implicated in the early post-training period was subsequently confirmed with activation and nuclear translocation seen in dentate granule neurons following training. mRNA changes for four genes, LRP3 (0 h), alpha actin (3 h), SNAP25 and NSF (6-12 h), were validated at message and/or protein level and shown to be learning specific. Thus, the memory-associated transcriptional cascade supports the cardinal periods of synaptic loosening, reorganisation and selection thought to underpin the process of long-term memory consolidation in the hippocampus.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> University College Dublin -> PubMed
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

Keith J. Murphy, Ciaran M Regan, Desmond G Higgins, Paul N Moynagh, John J O'Connor, Lisa Conboy, Mark Pickering, Graham K. Sheridan, Paul A. McGettigan, Niamh C O'Sullivan

Experts in our system

1
Keith J. Murphy
University College Dublin
Total Publications: 37
 
2
Desmond G Higgins
University College Dublin
Total Publications: 76
 
3
Paul N. Moynagh
Maynooth University
Total Publications: 91
 
4
John J O'Connor
University College Dublin
Total Publications: 57
 
5
Graham K. Sheridan
Trinity College Dublin
Total Publications: 10
 
6
Paul A. McGettigan
University College Dublin
Total Publications: 46