Type

Journal Article

Authors

E Gormley
D P Sleeman
D O'Meara
S Lesellier
E. Costello
L A L Corner

Subjects

Veterinary

Topics
tuberculosis animal diseases mustelidae animals female gastrointestinal tract microbiology lymph nodes pathology veterinary male mycobacterium bovis lung

Experimental tuberculosis in the European badger (Meles meles) after endobronchial inoculation of Mycobacterium bovis: I. Pathology and bacteriology. (2006)

Abstract The aim was to develop an endobronchial infection procedure for the study of Mycobacterium bovis infection in badgers. The badgers were anaesthetised and a cannula was passed per os to the tracheal bifurcation. When in place 1 ml of M. bovis suspension was inoculated. Three concentrations of M. bovis suspension were used; <10 colony forming units (cfu), approximately 10(2) cfu and approximately 3 x 10(3) cfu. The badgers were examined at three weekly intervals for clinical signs of disease and a tracheal aspirate was collected at each examination. The badgers were euthanased 17 weeks post infection (pi) and at the post mortem examination a wide range of tissues were examined for gross and histopathological lesions of tuberculosis and cultured for M. bovis. A sample of bronchial alveolar lavage (BAL) fluid was collected at post mortem for culture. At post mortem examination 17 weeks after infection, gross and histopathological lesions of tuberculosis were observed in all badgers inoculated with the high and medium dose and 1/3 inoculated with the low dose. M. bovis was recovered from all inoculated badgers. Infection in the high dose group was more widely disseminated than in the other groups. The number of sites with gross and histopathological lesions increased with increasing dose of M. bovis. All tracheal aspirates were negative on culture and only one BAL, collected from a badger of the high dose group, was positive on culture. No clinical signs due to the experimental infection were observed. The endobronchial route of inoculation is an effective route for establishing experimental infection, and could be used for studies of tuberculosis pathogenesis, immunology of M. bovis infection in badgers and for challenging badgers in vaccine protection studies. Badgers appeared to be very susceptible to infection by this procedure even with a dose of < 10 cfu but appear to control and limit the resulting infection.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

E Gormley, D P Sleeman, D O'Meara, S Lesellier, E. Costello, L A L Corner

Experts in our system

1
E Gormley
University College Dublin
Total Publications: 68
 
2
Sandrine Lesellier
University College Dublin
Total Publications: 14
 
3
Eamon Costello
Dublin City University
Total Publications: 94
 
4
L A L Corner
University College Dublin
Total Publications: 34