Type

Journal Article

Authors

Finian Martin
Madeline Murphy
Darren Finlay
Teresa Lambe

Subjects

Biochemistry

Topics
mice cells cultured nucleic acid hybridization mammary glands animal sexual maturation growth development cell proliferation connexins metabolism rna messenger connexin 43alpha1 mouse cell differentiation gene expression animals female apoptosis epithelial cells pregnancy

Differential expression of connexin 43 in mouse mammary cells. (2005)

Abstract In this study we have employed suppressive subtractive hybridization (SSH) analysis to investigate differential gene expression in primary mouse mammary epithelial cells (PMMEC) cultured under mildly apoptotic/quiescent and differentiating conditions. Among a small group of genes whose expression was differentially regulated was connexin 43. In vitro, connexin 43 mRNA and protein were detectable in PMMEC cultured under proliferative or mildly apoptotic conditions. The level of connexin 43 mRNA expression in vivo was also investigated. High levels of expression were found to be associated with the periods of greatest glandular plasticity (pubertal expansion of the mammary tree, early pregnancy and during early involution). Thus, terminally differentiated cells in vivo and in vitro did not express connexin 43 mRNA suggesting that connexin 43 expression, and perhaps facilitated gap junction communication, is associated with undifferentiated progenitor cell populations.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Finian Martin, Madeline Murphy, Darren Finlay, Teresa Lambe

Experts in our system

1
Finian Martin
University College Dublin
Total Publications: 81
 
2
Madeline Murphy
University College Dublin
Total Publications: 37