Type

Journal Article

Authors

P. Lonergan
A.C.O. Evans
M P Boland
J J Ireland
P M Coussens
G W Smith
O V Patel
D Rizos
S Park
T Fair
and 1 others

Subjects

Biochemistry

Topics
pregnancy female cattle molecular sequence data genetics animals transcription genetic in situ hybridization gene expression profiling rna messenger embryonic development base sequence dna primers blastocyst gene expression regulation developmental physiology expressed sequence tags fertilization in vitro oligonucleotide array sequence analysis protein biosynthesis reverse transcriptase polymerase chain reaction

Suppressed expression of genes involved in transcription and translation in in vitro compared with in vivo cultured bovine embryos. (2006)

Abstract In vivo-derived bovine embryos are of higher quality than those derived in vitro. Many of the differences in quality can be related to culture environment-induced changes in mRNA abundance. The aim of this study was to identify a range of mRNA transcripts that are differentially expressed between bovine blastocysts derived from in vitro versus in vivo culture. Microarray (BOTL5) comparison between in vivo- and in vitro-cultured bovine blastocysts identified 384 genes and expressed sequence tags (ESTs) that were differentially expressed; 85% of these were down-regulated in in vitro cultured blastocysts, showing a much reduced overall level of mRNA expression in in vitro- compared with in vivo-cultured blastocysts. Relative expression of 16 out of 23 (70%) differentially expressed genes (according to P value) were verified in new pools of in vivo- and in vitro-cultured blastocysts, using quantitative real-time PCR. Most (10 out of 16) are involved in transcription and translation events, suggesting that the reason why in vitro-derived embryos are of inferior quality compared with in vivo-derived embryos is due to a deficiency of the machinery associated with transcription and translation.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

P. Lonergan, A.C.O. Evans, M P Boland, J J Ireland, P M Coussens, G W Smith, O V Patel, D Rizos, S Park, T Fair and 1 others

Experts in our system

1
P. Lonergan
University College Dublin
Total Publications: 190
 
2
A.C.O. Evans
University College Dublin
Total Publications: 92
 
3
M P Boland
University College Dublin
Total Publications: 103
 
4
D Rizos
University College Dublin
Total Publications: 42
 
5
Stephen D. E. Park
University College Dublin
Total Publications: 35
 
6
T Fair
University College Dublin
Total Publications: 66