Type

Journal Article

Authors

B.P. Mahon
J. Brady
J.P. Cassidy
O. Zachariadis

Subjects

Microbiology

Topics
bordetella infections inflammatory response etiology gamma delta t cells microbiology institute of immunology immunology t lymphocyte subsets animals bronchoalveolar lavage fluid bordetella pertussis respiratory infection acute respiratory distress syndrome mice receptors antigen t cell gamma delta respiratory tract infections mice inbred c57bl gene expression regulation bacterial inflammation physiology pathology physiopathology biology

gammadelta T cells regulate the early inflammatory response to bordetella pertussis infection in the murine respiratory tract. (2006)

Abstract The role of gammadelta T cells in the regulation of pulmonary inflammation following Bordetella pertussis infection was investigated. Using a well-characterized murine aerosol challenge model, inflammatory events in mice with targeted disruption of the T-cell receptor delta-chain gene (gammadelta TCR-/- mice) were compared with those in wild-type animals. Early following challenge with B. pertussis, gammadelta TCR-/- mice exhibited greater pulmonary inflammation, as measured by intra-alveolar albumin leakage and lesion histomorphometry, yet had lower contemporaneous bacterial lung loads. The larger numbers of neutrophils and macrophages and the greater concentration of the neutrophil marker myeloperoxidase in bronchoalveolar lavage fluid from gammadelta TCR-/- mice at this time suggested that differences in lung injury were mediated through increased leukocyte trafficking into infected alveoli. Furthermore, flow cytometric analysis found the pattern of recruitment of natural killer (NK) and NK receptor+ T cells into airspaces differed between the two mouse types over the same time period. Taken together, these findings suggest a regulatory influence for gammadelta T cells over the early pulmonary inflammatory response to bacterial infection. The absence of gammadelta T cells also influenced the subsequent adaptive immune response to specific bacterial components, as evidenced by a shift from a Th1 to a Th2 type response against the B. pertussis virulence factor filamentous hemagglutinin in gammadelta TCR-/- mice. The findings are relevant to the study of conditions such as neonatal B. pertussis infection and acute respiratory distress syndrome where gammadelta T cell dysfunction has been implicated in the inflammatory process.
Collections Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes: Institute of Immunology
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Biology
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering: Research Institutes
Ireland -> University College Dublin -> PubMed
Ireland -> Maynooth University -> Type = Article
Ireland -> Maynooth University -> Academic Unit = Faculty of Science and Engineering
Ireland -> Maynooth University -> Status = Published
Ireland -> Maynooth University -> Open Access DRIVERset

Full list of authors on original publication

B.P. Mahon, J. Brady, J.P. Cassidy, O. Zachariadis

Experts in our system

1
Bernard P. Mahon
Maynooth University
Total Publications: 73
 
2
Jennifer J Brady
University College Dublin
Total Publications: 10
 
3
Joseph P. Cassidy
University College Dublin
Total Publications: 55