Journal Article


Wouter Duetz
Alan D W Dobson
Julian R Marchesi
Niall P O'Leary
Kevin E O'Connor



soil microbiology bacteria sequence analysis dna isolation purification phenylacetic acid biodegradation environmental phenylacetates genetics biodiversity metabolism rna ribosomal 16s oxygen phylogeny dna bacterial

Isolation and characterization of a diverse group of phenylacetic acid degrading microorganisms from pristine soil. (2004)

Abstract A diverse range of microorganisms capable of growth on phenylacetic acid as the sole source of carbon and energy were isolated from soil. Sixty six different isolates were identified and grouped according to 16S rRNA gene RFLP analysis. Subsequent sequencing of 16S rDNA from selected strains allowed further characterization of the phenylacetic acid degrading population isolated from soil. Nearly half (30) of the isolates are Bacillus species while the rest of the isolates are strains from a variety of genera namely, Arthrobacter, Pseudomonas, Rhodococcus, Acinetobacter, Enterobacter, Flavobacterium, and Paenibacillus. Sixty-one of the sixty-six strains reproducibly grew in defined minimal liquid culture medium (E2). All strains isolated grew when at least one hydroxylated derivative of phenylacetic acid was supplied as the carbon source, while 59 out of the 61 strains tested, accumulated ortho-hydroxyphenylacetic acid in the assay buffer; when pulsed with phenylacetic acid. Oxygen consumption experiments failed to indicate a clear link between phenylacetic acid and hydroxy-substituted phenylacetic acid in isolates from a broad range of genera.
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Full list of authors on original publication

Wouter Duetz, Alan D W Dobson, Julian R Marchesi, Niall P O'Leary, Kevin E O'Connor

Experts in our system

Alan D W Dobson
University College Cork
Total Publications: 116
Julian R Marchesi
University College Cork
Total Publications: 45
Kevin E O'Connor
University College Dublin
Total Publications: 52