Type

Journal Article

Authors

M J Duffy
N O'Higgins
E McDermott
A D K Hill
J Crown
C O'Shea
B Browne
N O'Donovan
B Ryan

Subjects

Biochemistry

Topics
alternative splicing inhibitor of apoptosis proteins metabolism humans birc5 protein human breast female apoptosis neoplasm proteins pathology microtubule associated proteins breast neoplasms fibroadenoma

Expression of survivin and its splice variants survivin-2B and survivin-DeltaEx3 in breast cancer. (2004)

Abstract Alternative splicing of survivin mRNA gives rise to multiple isoforms, that is, survivin and 3 splice variants, survivin-2B, survivin-3B and survivin-DeltaEx3. The aim of this study was to compare the expression of survivin, survivin-2B and survivin-DeltaEx3 in normal breast tissue, fibroadenomas, primary breast cancer and axillary nodal metastases. Survivin, survivin-2B and survivin-DeltaEx3 mRNA were measured using semiquantitative RT-PCR. In the primary carcinomas, we related mRNA for each form of survivin to both survivin protein and apoptosis. For each type of breast tissue, survivin was the predominant form detected, being present in 146 out of 156 (93.6%) primary breast carcinomas, 11 out of 11 (100%) axillary nodal metastases, 21 out of 31 (67.7%) fibroadenomas and five out of 22 (22.7%) specimens of normal breast tissue. Levels of the three forms of survivin were significantly higher in the carcinomas compared to normal breast tissue (P < 0.0001). Levels of both survivin-2B and survivin-DeltaEx3 but not survivin were significantly higher in nodal metastases than primary carcinomas. Survivin mRNA levels correlated significantly with survivin protein. Finally, both survivin and survivin-DeltaEx3 but not survivin-2B correlated positively with apoptosis. Although survivin, survivin-2B and survivin-DeltaEx3 were all detected in both malignant and nonmalignant breast tissue, the predominant form was survivin. Our results suggest that the different forms of survivin may have different roles in apoptosis in breast cancer.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M J Duffy, N O'Higgins, E McDermott, A D K Hill, J Crown, C O'Shea, B Browne, N O'Donovan, B Ryan

Experts in our system

1
Michael J Duffy
University College Dublin
 
2
E W McDermott
University College Dublin
Total Publications: 49
 
3
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
 
4
John Crown
Dublin City University
Total Publications: 104
 
5
C J O'Shea
University College Dublin
 
6
Brigid C Browne
Dublin City University
Total Publications: 11
 
7
Norma O'Donovan
Dublin City University
Total Publications: 59