Type

Journal Article

Authors

M P Boland
T Fair
A Gutiérrez-Adán
D Rizos
P. Lonergan

Subjects

Veterinary

Topics
standards blastocyst veterinary fertilization cytology fertilization in vitro humans physiology embryo mammalian gene expression organ culture techniques pregnancy multiple animals pregnancy female oocytes cattle

Effect of culture environment on embryo quality and gene expression - experience from animal studies. (2003)

Abstract Recent studies comparing bovine oocyte maturation, fertilization and embryo culture in vivo and in vitro have demonstrated that the origin of the oocyte is the main factor affecting blastocyst yield, while the post-fertilization culture environment is critical in determining blastocyst quality, measured in terms of cryotolerance and relative transcript abundance, irrespective of the origin of the oocyte. Production of embryos in vitro, particularly when using an extended period of in-vitro culture, may predispose the embryo to phenomena such as the large offspring syndrome, which is likely to alter gene expression, particularly of imprinted genes. It is clear now that the post-fertilization culture environment has a profound effect on the relative abundance of gene transcripts within the embryo, and culture under suboptimal conditions for as little as 1 day can lead to perturbations in the pattern of expression.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M P Boland, T Fair, A Gutiérrez-Adán, D Rizos, P. Lonergan

Experts in our system

1
M P Boland
University College Dublin
Total Publications: 103
 
2
T Fair
University College Dublin
Total Publications: 66
 
3
D Rizos
University College Dublin
Total Publications: 42
 
4
P. Lonergan
University College Dublin
Total Publications: 190