Journal Article


P Newsholme
P R Flatt
N McClenaghan
J Nolan
G Dixon



cell line alanine drug effects physiology dose response relationship drug glutamic acid pharmacology animals clone cells glucose glutamine insulin female rats rats wistar metabolism adenosine triphosphate islets of langerhans

A comparative study of amino acid consumption by rat islet cells and the clonal beta-cell line BRIN-BD11 - the functional significance of L-alanine. (2003)

Abstract Evidence has been published that L -alanine may, under appropriate conditions, promote insulin secretion in normal rodent islets and various beta cell lines. Previous results utilising the clonal beta-cell line BRIN-BD11, demonstrated that alanine dramatically elevated insulin release by a mechanism requiring oxidative metabolism. We demonstrate in this paper that addition ofL -alanine had an insulinotropic effect in dispersed primary islet cells. Addition of D -glucose increasedL -alanine consumption in both BRIN-BD11 cells and primary islet cells.L -glutamine consumption in the BRIN-BD11 cell line and primary rat islets was also determined. The consumption rate was in line with that previously reported for cells of the immune system and other glutamine-utilising cells or tIssues. However,L -alanine consumption was at least an order of magnitude higher thanL -glutamine consumption. The metabolism ofL -alanine in the beta-cell may result in stimulation of insulin secretion via generation of metabolic stimulus secretion coupling factors such asL -glutamate.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

P Newsholme, P R Flatt, N McClenaghan, J Nolan, G Dixon

Experts in our system

Philip Newsholme
University College Dublin
Total Publications: 51