Type

Journal Article

Authors

P Newsholme
P R Flatt
N McClenaghan
J Nolan
G Dixon

Subjects

Pharmacology

Topics
cell line alanine drug effects physiology dose response relationship drug glutamic acid pharmacology animals clone cells glucose glutamine insulin female rats rats wistar metabolism adenosine triphosphate islets of langerhans

A comparative study of amino acid consumption by rat islet cells and the clonal beta-cell line BRIN-BD11 - the functional significance of L-alanine. (2003)

Abstract Evidence has been published that L -alanine may, under appropriate conditions, promote insulin secretion in normal rodent islets and various beta cell lines. Previous results utilising the clonal beta-cell line BRIN-BD11, demonstrated that alanine dramatically elevated insulin release by a mechanism requiring oxidative metabolism. We demonstrate in this paper that addition ofL -alanine had an insulinotropic effect in dispersed primary islet cells. Addition of D -glucose increasedL -alanine consumption in both BRIN-BD11 cells and primary islet cells.L -glutamine consumption in the BRIN-BD11 cell line and primary rat islets was also determined. The consumption rate was in line with that previously reported for cells of the immune system and other glutamine-utilising cells or tIssues. However,L -alanine consumption was at least an order of magnitude higher thanL -glutamine consumption. The metabolism ofL -alanine in the beta-cell may result in stimulation of insulin secretion via generation of metabolic stimulus secretion coupling factors such asL -glutamate.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

P Newsholme, P R Flatt, N McClenaghan, J Nolan, G Dixon

Experts in our system

1
Philip Newsholme
University College Dublin
Total Publications: 51