Journal Article


P Newsholme
P R Flatt
N H McClenaghan
J P G Malthouse
C Hewage
M Corless
L Brennan



drug effects magnetic resonance spectroscopy glutamic acid models biological pharmacology gamma glutamylcyclotransferase glucose glutamine clone cells aspartic acid animals metabolism islets of langerhans carbon isotopes

13C NMR analysis reveals a link between L-glutamine metabolism, D-glucose metabolism and gamma-glutamyl cycle activity in a clonal pancreatic beta-cell line. (2003)

Abstract Pancreatic islet cells and clonal beta-cell lines can metabolise L-glutamine at high rates. The pathway of L-glutamine metabolism has traditionally been described as L-glutamine-->L-glutamate-->2-oxoglutarate-->oxidation in TCA cycle following conversion to pyruvate. Controversially, the metabolism of D-glucose to L-glutamate in beta cells is not widely accepted. However, L-glutamate has been proposed to be a stimulation-secretion coupling factor in glucose-induced insulin secretion. We aimed to investigate the metabolism of glutamine and glucose by using (13)C NMR analysis. BRIN-BD11 cells were incubated in the presence of 16.7 mmol/l [1-(13)C]glucose, 2 mmol/l [2-(13)C]L-glycine or 2 mmol/l [1,2-(13)C]glutamine in the presence or absence of other amino acids or inhibitors. After an incubation period the cellular metabolites were extracted using a PCA extract procedure. After neutralisation, the extracts were prepared for analysis using (13)C-NMR spectroscopy. Using (13)C NMR analysis we have shown that L-glutamine could be metabolised in BRIN-BD11 cells via reactions constituting part of the gamma-glutamyl cycle producing glutathione. Moderate or high activities of the enzymes required for these pathways of metabolism including glutaminase, gamma-glutamyltransferase and gamma-glutamylcysteine synthetase were observed. We additionally report significant D-glucose metabolism to L-glutamate. Addition of the aminotransferase inhibitor, aminooxyacetate, attenuated L-glutamate production from D-glucose. We propose that L-glutamine metabolism is important in the beta cell for generation of stimulus-secretion coupling factors, precursors of glutathione synthesis and for supplying carbon for oxidation in the TCA cycle. D-glucose, under appropriate conditions, can be converted to L-glutamate via an aminotransferase catalysed step.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

P Newsholme, P R Flatt, N H McClenaghan, J P G Malthouse, C Hewage, M Corless, L Brennan

Experts in our system

Philip Newsholme
University College Dublin
Total Publications: 51
J.Paul G. Malthouse
University College Dublin
Total Publications: 71
Lorraine Brennan
University College Dublin
Total Publications: 166