Type

Journal Article

Authors

M P Boland
T Fair
A Gutiérrez-Adán
D Rizos
P. Lonergan

Subjects

Biochemistry

Topics
animals embryology female embryo transfer oocytes cattle pregnancy genetics veterinary cell survival cells cultured embryonic and fetal development fertilization in vitro blastocyst cytology physiology gene expression embryo mammalian

Oocyte and embryo quality: effect of origin, culture conditions and gene expression patterns. (2003)

Abstract In general, the majority of immature bovine oocytes fail to develop to the blastocyst stage following maturation, fertilization and culture in vitro. The evidence suggests that while culture conditions during in vitro embryo production can impact on the developmental potential of the early embryo, the intrinsic quality of the oocyte is the key factor determining the proportion of oocytes developing to the blastocyst stage. In addition, evidence suggests that the period of post-fertilization embryo culture is the most critical in determining blastocyst quality. This paper reviews the current literature, with emphasis on the bovine model, demonstrating evidence for an effect of oocyte origin and/or in vitro maturation conditions on the developmental capacity and gene expression patterns in the oocyte. Furthermore, the well-documented effects of post-fertilization culture environment on embryo gene expression and quality are highlighted.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M P Boland, T Fair, A Gutiérrez-Adán, D Rizos, P. Lonergan

Experts in our system

1
M P Boland
University College Dublin
Total Publications: 103
 
2
T Fair
University College Dublin
Total Publications: 66
 
3
D Rizos
University College Dublin
Total Publications: 42
 
4
P. Lonergan
University College Dublin
Total Publications: 190