Type

Journal Article

Authors

W M Gallagher
W S Argraves
P A Dervan
A H McCann
N J O'Higgins
A D Hill
E W McDermott
M J Duffy
W O Twal
L M Greene

Subjects

Microbiology

Topics
fibulin immunohistochemistry receptors estrogen antibodies monoclonal female genetics carcinoma metabolism breast calcium binding proteins biosynthesis pathology breast neoplasms humans physiology diagnostic use

Elevated expression and altered processing of fibulin-1 protein in human breast cancer. (2003)

Abstract The extracellular matrix protein fibulin-1 suppresses the motility and invasiveness of a variety of tumour cell types in vitro as well as the growth of fibrosarcoma tumours in nude mice. In this study, fibulin-1 protein expression in breast carcinoma specimens and normal breast tissue was evaluated immunohistologically. Fibulin-1 protein expression was also semiquantitatively assessed by immunoblot analysis in a collection of normal breast tissues (n=18), benign tumours (n=5) and breast carcinomas (n=39). In normal breast tissue, fibulin-1 protein expression predominated in the ductal epithelium and underlying myoepithelium, with weaker staining evident in the loose connective surrounding the ducts. Examination of breast carcinomas revealed that the tumour cells also expressed fibulin-1 protein. The level of mature fibulin-1 polypeptide (100 kDa) was higher in the breast carcinoma specimens as compared to normal breast tissue (Mann-Whitney U-test, P=0.0005). In addition to the mature fibulin-1 polypeptide, several smaller sized polypeptides of 55, 50 and 25 kDa were detected using monoclonal antibodies reactive towards an epitope located at the N-terminus of fibulin-1. The immunoreactive 50 kDa polypeptide was detected more frequently in breast carcinoma specimens than in normal breast tissue (chi(2)=17.22, P<0.0001). Furthermore, the ratio of the 50 kDa fragment to the mature fibulin-1 polypeptide correlated with the level of oestrogen receptor alpha (Spearman correlation coefficient, rs=0.49, P<0.003, n=36) and progesterone receptor (rs=0.43, P=0.008, n=36) expression in the tumour specimens. Taken together, these findings indicate that elevated expression and altered processing of fibulin-1 is associated with human breast cancer.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

W M Gallagher, W S Argraves, P A Dervan, A H McCann, N J O'Higgins, A D Hill, E W McDermott, M J Duffy, W O Twal, L M Greene

Experts in our system

1
William M Gallagher
University College Dublin
Total Publications: 148
 
2
Amanda McCann
University College Dublin
Total Publications: 41
 
3
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
 
4
E W McDermott
University College Dublin
Total Publications: 49
 
5
Michael J Duffy
University College Dublin