Type

Journal Article

Authors

Michael J Duffy
Niall O'Higgins
Enda McDermott
Arnold D K Hill
Helen Stunell
John Crown
Norma O'Donovan

Subjects

Biochemistry

Topics
fibroadenoma breast neoplasms casp3 protein human transcription genetic caspases pathology receptors estrogen humans metabolism rna messenger receptors progesterone enzyme precursors carcinoma gene expression regulation enzymologic caspase 3 reverse transcriptase polymerase chain reaction female gene expression regulation neoplastic genetics apoptosis

Caspase 3 in breast cancer. (2003)

Abstract An inability to undergo apoptosis is widely thought to contribute to both tumorigenesis and tumor progression. One of the key mediators of apoptosis is the thiol protease caspase 3. In this investigation, caspase 3 mRNA and protein expression in breast cancer was examined. Caspase 3 was measured at the mRNA level using reverse transcription-PCR and at the protein level using both Western blotting and activity assays. Levels of apoptosis were determined using an ELISA, which detects nucleosomes released during DNA fragmentation. Relative levels of caspase 3 mRNA were similar in breast carcinomas (n = 103), fibroadenomas (n = 25), and normal breast tissues (n = 6). However, levels of both the precursor and active forms of caspase 3 were significantly higher in carcinomas compared with both fibroadenomas (P = 0.0188) and normal breast tissues (P = 0.0002). Levels of apoptosis were also highest in the carcinomas and correlated significantly with active caspase 3 levels (r = 0.481; P = 0.0003). In the carcinomas, expression of caspase 3 showed no significant relationship with either tumor size, tumor grade, nodal status, or steroid receptor status but was significantly higher in ductal carcinomas than in lobular carcinomas (P = 0.0188). We conclude that rates of apoptosis as measured by both caspase 3 activation and nucleosome release are higher in breast cancer than in nonmalignant breast tissue. This finding would appear to conflict with the widely held belief that apoptosis is reduced in malignancy. The proliferation:apoptosis ratio, however, may be higher in carcinomas than in the corresponding normal tissue.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Michael J Duffy, Niall O'Higgins, Enda McDermott, Arnold D K Hill, Helen Stunell, John Crown, Norma O'Donovan

Experts in our system

1
Michael J Duffy
University College Dublin
 
2
E W McDermott
University College Dublin
Total Publications: 49
 
3
Arnold D K Hill
Royal College of Surgeons in Ireland
Total Publications: 110
 
4
John Crown
Dublin City University
Total Publications: 104
 
5
Norma O'Donovan
Dublin City University
Total Publications: 59