Type

Journal Article

Authors

Patrick Lonergan
Maurice Boland
Jan Motlik
Poul Hyttel
Trudee Fair

Subjects

Biochemistry

Topics
cell nucleolus pharmacology cytology nuclear proteins drug effects physiology ultrastructure analogs derivatives female protein kinase inhibitors ovarian follicle cell size meiosis cattle butyrolactone i animals oocytes 4 butyrolactone metabolism

Maintenance of meiotic arrest in bovine oocytes in vitro using butyrolactone I: effects on oocyte ultrastructure and nucleolus function. (2002)

Abstract In this study, we have shown that butyrolactone I (BL-I), a potent inhibitor of cyclin-dependent kinases, affects oocyte cytoplasmic morphology and nuclear function in terms of nucleolar ultrastructure and immunocytochemistry. Bovine oocytes were recovered from three classes of follicle size: 1.5-2, 2-3, and 3-6 mm. The oocytes were incubated for 40 hr with BL-I, and subsequently processed for transmission electron microscopy or immunocytochemistry. A control group of oocytes were processed immediately upon recovery (0 hr). In general, incubation with BL-I for 40 hr disrupted contact between cells of the cumulus oophorous and the oocyte, caused degeneration of the cortical granules and the peripheral migration of all cytoplasmic organelles. At the level of the nucleus, it induced convolution of the nuclear membrane and caused acceleration of nucleolar compaction in oocytes from follicles < 3 mm and fragmentation of nucleoli, particularly evidenced by immunocytochemistry, in oocytes from follicles > 3 mm. Furthermore, the effects appear to be more profound in fully-grown oocytes.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Patrick Lonergan, Maurice Boland, Jan Motlik, Poul Hyttel, Trudee Fair

Experts in our system

1
P. Lonergan
University College Dublin
Total Publications: 190
 
2
M P Boland
University College Dublin
Total Publications: 103
 
3
T Fair
University College Dublin
Total Publications: 66