Type

Journal Article

Authors

M P Boland
F Ward
D Rizos
P. Lonergan

Subjects

Veterinary

Topics
cell survival standards cells cultured embryonic and fetal development fertilization in vitro blastocyst reproductive techniques cytology physiology embryo mammalian animals embryology female embryo transfer oocytes cattle pregnancy cryopreservation veterinary

Factors influencing oocyte and embryo quality in cattle. (2002)

Abstract Following in vitro maturation, approximately 90% of immature bovine oocytes will reach metaphase II and extrude the first polar body; approximately 80% will undergo fertilization and cleave, at least once, to the two-cell stage. However, only about 30-40% will ever reach the blastocyst stage. This would suggest that the post-fertilization part of the process of in vitro embryo production, the longest part, is the main period determining blastocyst yield. The experiments described in this paper clearly demonstrate that this is. in fact, not the case and that it is events further back along the developmental axis that determine the proportion of immature oocytes reaching the blastocyst stage. The results also show, however, that the post-fertilization culture period is of profound importance in determining the equality of those blastocysts that do develop, with those produced in vitro consistently being of inferior quality to their in vivo produced conterparts. The challenge for the future is to modify our conditions of post-fertilization embryo culture in an attempt to mimic those that occur naturally in vivo and in that way improve blastocyst quality.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M P Boland, F Ward, D Rizos, P. Lonergan

Experts in our system

1
M P Boland
University College Dublin
Total Publications: 103
 
2
D Rizos
University College Dublin
Total Publications: 42
 
3
P. Lonergan
University College Dublin
Total Publications: 190