Type

Journal Article

Authors

M P Boland
F A Ward
P Hyttel
D C Cottell
A Dinnyes
P. Lonergan
T Fair

Subjects

Veterinary

Topics
glycerol animals female veterinary blastocyst cryoprotective agents cattle ultrastructure cryopreservation

Ultrastructure of bovine blastocysts following cryopreservation: effect of method of blastocyst production. (2001)

Abstract The objective of this study was to describe the ultrastructure of blastocysts derived by in vivo and in vitro methods and to investigate how the morphology is affected by exposure to cryoprotectant (10% glycerol) or cryopreservation by conventional slow freezing. In vivo derived blastocysts were characterized by a narrow perivitelline space (PvS), a continuous cover of numerous stacked microvilli (MV) on the plasma membrane, a well-defined system of cell-to-cell coupling and a large population of round or elongated mitochondria with numerous transverse cristae. Exposure of these blastocysts to cryoprotectant was manifested by shrinkage of the blastocysts and swelling of the mitochondria. Cryopreservation resulted in further shrinkage, damage to the MV, and accumulation of cellular debris. In comparison, the in vitro matured (IVM)/in vitro fertilized (IVF) in vivo cultured blastocysts displayed a wider PvS; they appeared to possess less MV and all blastocysts displayed some cellular debris in their PvS. There was also a decrease in the number of junctional contacts between the trophoblastic cells. The reaction of these blastocysts to exposure to cryoprotectant was similar to that of the in vivo derived blastocysts. However, they appeared to be more susceptible to cryopreservation. The totally in vitro produced (IVP) blastocysts displayed a wider PvS, no stacking of the MV, increased numbers of lipid droplets and a further reduction in the junctional contacts between trophoblastic cells. The IVP blastocysts sustained breakage of the zona pellucida on exposure to cryoprotectant and were extremely sensitive to cryopreservation, losing all cell structure and organization. The findings of the present study indicate that in vivo derived blastocysts possess certain structural characteristics that confer a greater tolerance on them to exposure to cryoprotectant and cryopreservation.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

M P Boland, F A Ward, P Hyttel, D C Cottell, A Dinnyes, P. Lonergan, T Fair

Experts in our system

1
M P Boland
University College Dublin
Total Publications: 103
 
2
P. Lonergan
University College Dublin
Total Publications: 190
 
3
T Fair
University College Dublin
Total Publications: 66