Journal Article


M Boland
X Yang
T Fair
A Dinnyes
P. Lonergan



drug effects male enzyme inhibitors growth development analogs derivatives antagonists inhibitors embryonic and fetal development oocytes pharmacology cyclin dependent kinases female meiosis cattle butyrolactone i animals 4 butyrolactone

Bovine oocyte and embryo development following meiotic inhibition with butyrolactone I. (2000)

Abstract In this study we have shown that butyrolactone I (BL-I), a potent inhibitor of cyclin-dependent kinases, inhibits meiotic resumption in bovine oocytes by blocking germinal vesicle breakdown in a dose-dependent manner. A concentration 100 microM blocked over 60% of oocytes, while 150 microM inhibited almost all oocytes compared to the control in which over 80% resumed meiosis. Following a second 24 hr culture under conditions permissive to normal maturation, almost all (95%) of blocked oocytes resumed meiosis and progressed to metaphase II. In terms of developmental competence, oocytes maintained in meiotic arrest for 24 hr with 100 microM exhibited a similar capacity to develop to the blastocyst stage as nonblocked control oocytes following maturation, fertilization, and culture in vitro. Cryopreservation was employed as a tool to detect differences in the oocyte viability between blocked and control oocytes. Cleavage of oocytes was significantly reduced following vitrification and activation both in BL-I treated (40.2% vs. 71.9%, P < 0.05) and the control groups (45.6% vs. 81.7%, P < 0.05). However, BL-I treated oocytes were less likely to develop into blastocysts following vitrification (20.0% from vitrified vs 42.5% from nonvitrified cleaved oocytes, P < 0.05, based on cleaved oocytes) compared to nontreated oocytes (34.0% from vitrified vs. 42. 9% from nonvitrified oocytes, P < 0.05). These results demonstrate the feasibility of maintaining bovine oocytes in artificial meiotic arrest without compromising their subsequent developmental competence and may represent a tool for improving the development of less competent oocytes.
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Full list of authors on original publication

M Boland, X Yang, T Fair, A Dinnyes, P. Lonergan

Experts in our system

M P Boland
University College Dublin
Total Publications: 103
T Fair
University College Dublin
Total Publications: 71
P. Lonergan
University College Dublin
Total Publications: 207