Type

Journal Article

Authors

Colin Hill
R. Paul Ross
Stephen R. Stockdale
Fabien J. Cousin
Ronan Strain
Horst Neve
Eric De Haas
Marion Dalmasso

Subjects

Microbiology

Topics
ultrastructure bacterial biofilms saliva humans physiology bacteriophages live cells antimicrobials microscopy electron transmission surface isolation molecular sequence data human saliva artificial biofilms phage virology streptococcus mutans streptococcus phages

Isolation of a Novel Phage with Activity against Streptococcus mutans Biofilms (2015)

Abstract Streptococcus mutans is one of the principal agents of caries formation mainly, because of its ability to form biofilms at the tooth surface. Bacteriophages (phages) are promising antimicrobial agents that could be used to prevent or treat caries formation by S. mutans. The aim of this study was to isolate new S. mutans phages and to characterize their antimicrobial properties. A new phage, ɸAPCM01, was isolated from a human saliva sample. Its genome was closely related to the only two other available S. mutans phage genomes, M102 and M102AD. ɸAPCM01 inhibited the growth of S. mutans strain DPC6143 within hours in broth and in artificial saliva at multiplicity of infections as low as 2.5x10-5. In the presence of phage ɸAPCM01 the metabolic activity of a S. mutans biofilm was reduced after 24 h of contact and did not increased again after 48 h, and the live cells in the biofilm decreased by at least 5 log cfu/ml. Despite its narrow host range, this newly isolated S. mutans phage exhibits promising antimicrobial properties.
Collections Ireland -> Teagasc -> Food Biosciences

Full list of authors on original publication

Colin Hill, R. Paul Ross, Stephen R. Stockdale, Fabien J. Cousin, Ronan Strain, Horst Neve, Eric De Haas, Marion Dalmasso

Experts in our system

1
Colin Hill
University College Cork
Total Publications: 351
 
2
R Paul Ross
Teagasc
Total Publications: 441
 
3
Stephen R Stockdale
University College Cork
Total Publications: 11
 
4
Fabien J. Cousin
University College Cork
Total Publications: 3
 
5
Horst Neve
Teagasc
 
6
Marion Dalmasso
Teagasc
Total Publications: 13