Type

Conference Proceedings

Authors

Fiona Regan
Richard O'Kennedy
Charles Nwankire
Caroline Murphy
Brendan Heery
Ivan Maguire
Jenny Fitzgerald

Subjects

Chemistry

Topics
lab on a disc microfluidics molecular biology biosensors novel the optical sensing high throughput fresh water

Novel one-step centrifugal sensor system for the detection of cyanobacterial toxin microcystin-LR (2015)

Abstract Globally, the most prevalent cyanobacterial toxins, in blooms from fresh and brackish waters, are the cyclic peptide toxins of the microcystin family. The need for on-site algal-toxin monitoring has become increasingly urgent due to the amplified demand for fresh-water and for safe, ‘toxin-free’ shellfish and fish stocks. Along with routine testing of shellfish stocks for biotoxins including microcystin, the EU also require routine monitoring for the presence of the causative algal species. Herein, we describe a novel, Lab-On-A-Disc (LOAD) platform which has been developed to assess microcystin toxin levels in-situ. Using recombinant antibody technology, the LOAD platform combines immunofluorescence with centrifugally driven microfluidic liquid handling to achieve a next-generation disposable device for high throughput sampling. A low-complexity, ‘LED-Photodiode’ based optical sensing system was tailor-made for the system, which allows the fluorescence signal of the microcystin-specific reaction to be quantified. This system can rapidly and accurately detect the presence of microcystin in less than 10 minutes with minimum user interaction and maximum reproducibility. This method provides a low cost diagnostic alternative to the current laborious and costly methods used for toxin monitoring.
Collections Ireland -> Dublin City University -> Publication Type = Conference or Workshop Item
Ireland -> Dublin City University -> Status = Unpublished
Ireland -> Dublin City University -> Subject = Biological Sciences: Microfluidics
Ireland -> Dublin City University -> DCU Faculties and Centres = Research Initiatives and Centres: Biomedical Diagnostics Institute (BDI)
Ireland -> Dublin City University -> Subject = Biological Sciences: Molecular biology
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Chemical Sciences
Ireland -> Dublin City University -> DCU Faculties and Centres = DCU Faculties and Schools: Faculty of Science and Health: School of Biotechnology
Ireland -> Dublin City University -> Subject = Biological Sciences: Biosensors

Full list of authors on original publication

Fiona Regan, Richard O'Kennedy, Charles Nwankire, Caroline Murphy, Brendan Heery, Ivan Maguire, Jenny Fitzgerald

Experts in our system

1
Fiona Regan
Dublin City University
Total Publications: 101
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Charles Nwankire
Dublin City University
Total Publications: 22
 
4
Caroline Murphy
Dublin City University
Total Publications: 12
 
5
Brendan Heery
Dublin City University
Total Publications: 31
 
6
Ivan Maguire
Dublin City University
Total Publications: 15
 
7
Jenny Fitzgerald
Dublin City University
Total Publications: 18