Type

Journal Article

Authors

Fiona Regan
Noel O'Connor
Dermot Brabazon
Gillian Duffy
Dian Zhang
Ciprian Briciu-Burghina
Brendan Heery

Subjects

Chemistry

Topics
rapid detection e coli sample site analysis surface sample preparation water site

ColiSense, today's sample today: A rapid on-site detection of β-d-Glucuronidase activity in surface water as a surrogate for E. coli. (2015)

Abstract A sensitive field-portable fluorimeter with incubating capability and triplicate sample chambers was designed and built. The system was optimised for the on-site analysis of E. coli in recreational waters using fluorescent based enzyme assays. The target analyte was β-d-Glucuronidase (GUS) which hydrolyses a synthetic substrate 6-Chloro-4-Methyl-Umbelliferyl-β-d-Glucuronide (6-CMUG) to release the fluorescent molecule 6-Chloro-4-Methyl-Umbelliferyl (6-CMU). The system was calibrated with 6-CMU standards. A LOD of 5nM and a resolution of less than 1nM was determined while enzyme kinetic tests showed detection of activities below 1pmolmin(-1)mL(-1) of sample. A field portable sample preparation, enzyme extraction protocol and continuous assay were applied with the system to analyse freshwater and marine samples. Results from a one day field trial are shown which demonstrated the ability of the system to deliver results on-site within a 75min period.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Fiona Regan, Noel O'Connor, Dermot Brabazon, Gillian Duffy, Dian Zhang, Ciprian Briciu-Burghina, Brendan Heery

Experts in our system

1
Fiona Regan
Dublin City University
Total Publications: 115
 
2
Noel E. O'Connor
Dublin City University
Total Publications: 474
 
3
Dermot Brabazon
Dublin City University
Total Publications: 162
 
4
Gillian Duffy
Dublin City University
Total Publications: 9
 
5
Dian Zhang
Dublin City University
Total Publications: 17
 
6
Ciprian Briciu-Burghina
Dublin City University
Total Publications: 6
 
7
Brendan Heery
Dublin City University
Total Publications: 33