Type

Journal Article

Authors

Robert J Forster
Richard O'Kennedy
Eadaoin Carthy
Kellie Adamson
Shikha Sharma
Sarah Gilgunn
Elaine Spain

Subjects

Medicine & Nursing

Topics
detection cancer diagnosis prostate specific antigen modified electrode prostate cancer self assembled monolayer monoclonal antibody pcr amplification

Detection of prostate specific antigen based on electrocatalytic platinum nanoparticles conjugated to a recombinant scFv antibody. (2015)

Abstract Highly sensitive and label free detection of prostate specific antigen (PSA) still remains a challenge in prostate cancer diagnosis. In this paper, we propose a sensitive electrochemical immunosensor based on electrocatalytic platinum nanoparticles conjugated to a recombinant scFv antibody. Gold disc electrodes functionalised with a l-Cysteine (Cys) self-assembled monolayer (SAM) were used to covalently bind PSA specific monoclonal antibody (anti-PSA) using N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. Immunosensing was completed using sandwich-type immunoreaction of the PSA-antigen (1-30ng/mL) between anti-PSA immobilized on the l-Cys modified electrode using label free electrochemical impedance (EIS) technique. Furthermore, highly specific in-house generated scFv fragments as receptor proteins were utilised for one step site-directed immobilisation on the surface of platinum nanoparticles (PtNPs). To improve the sensitivity of the immunoassay, these scFV labelled electrocatalytic PtNPs were then used for covalent hybridisation to the PSA modified electrode and then applied in a hybridisation assay to determine the concentration of the PSA by measuring the faradaic current associated with reduction of peroxide in solution. Semi-log plots of the PSA concentration vs. faradaic current are linear from 1 to 30ng/mL and pM concentrations can be detected without the need for molecular, e.g., PCR or NASBA, amplification.
Collections Ireland -> Dublin City University -> PubMed

Full list of authors on original publication

Robert J Forster, Richard O'Kennedy, Eadaoin Carthy, Kellie Adamson, Shikha Sharma, Sarah Gilgunn, Elaine Spain

Experts in our system

1
Robert J. Forster
Dublin City University
Total Publications: 90
 
2
R O'Kennedy
Dublin City University
Total Publications: 197
 
3
Kellie Adamson
Dublin City University
Total Publications: 7
 
4
Shikha Sharma
Dublin City University
Total Publications: 10
 
5
Elaine Spain
Dublin City University
Total Publications: 12