Type

Journal Article

Authors

Richard O'Kennedy
Hui Ma

Subjects

Microbiology

Topics
natural binding glycoproteins heavy chain chains recombinant antibodies single antibodies

The Structure of Natural and Recombinant Antibodies. (2015)

Abstract Immunoglobulins (Ig) isotypes A, D, E, G, and M are glycoproteins which are mainly composed of a "Y"-shaped Ig monomer (~150 kDa), consisting of two light and two heavy chains. Both light and heavy chains contain variable (N-terminal) and constant regions (C-terminal). Each light chain consists of one variable domain and one constant domain, whereas each heavy chain has one variable domain and three constant domains. However, heavy-chain antibodies consisting of only heavy chains and lacking the light chains are found in camelids and cartilaginous fishes. Unlike other immunoglobulins, the heavy chain of avian antibody IgY (~180 kDa) consists of four constant domains. The single-chain variable fragment (scFv; ~25 kDa) of an antibody contains variable regions of antibody heavy and light chains. The fragment antigen-binding (Fab; ~50 kDa) region has the full antibody light chain but the heavy chain is composed of a variable region and one constant domain.
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Full list of authors on original publication

Richard O'Kennedy, Hui Ma

Experts in our system

1
R O'Kennedy
Dublin City University
Total Publications: 197
 
2
Hui Ma
Dublin City University
Total Publications: 8