Journal Article


Kevin Kavanagh
Emer Reeves
Staffan Paulie
Christian Smedman
David A Bergin
Nessa Banville
Fred McMahon



neutrophils bacterial proteins neutrophil activation il 6 inflammatory cytokines neutrophil recruitment pro inflammatory crude protein

Activation of Neutrophils via IP3 Pathway Following Exposure to Demodex-Associated Bacterial Proteins. (2015)

Abstract Rosacea is a chronic inflammatory condition that predominantly affects the skin of the face. Sera from rosacea patients display elevated reactivity to proteins from a bacterium (Bacillus oleronius) originally isolated from a Demodex mite from a rosacea patient suggesting a possible role for bacteria in the induction and persistence of this condition. This work investigated the ability of B. oleronius proteins to activate neutrophils and demonstrated activation via the IP3 pathway. Activated neutrophils displayed increased levels of IP1 production, F-actin formation, chemotaxis, and production of the pro-inflammatory cytokines IL-1β and IL-6 following stimulation by pure and crude B. oleronius protein preparations (2 μg/ml), respectively. In addition, neutrophils exposed to pure and crude B. oleronius proteins (2 μg/ml) demonstrated increased release of internally stored calcium (Ca(2+)), a hallmark of the IP3 pathway of neutrophil activation. Neutrophils play a significant role in the inflammation associated with rosacea, and this work demonstrates how B. oleronius proteins can induce neutrophil recruitment and activation.
Collections Ireland -> Maynooth University -> PubMed

Full list of authors on original publication

Kevin Kavanagh, Emer Reeves, Staffan Paulie, Christian Smedman, David A Bergin, Nessa Banville, Fred McMahon

Experts in our system

Kevin Kavanagh
Maynooth University
Total Publications: 183
Emer P Reeves
Royal College of Surgeons in Ireland
Total Publications: 63
David A Bergin
Royal College of Surgeons in Ireland
Total Publications: 28
Nessa Banville
Maynooth University
Total Publications: 9