Type

Journal Article

Authors

Kevin E O'Connor
Jasmina Nikodinovic-Runic
Veselin Maslak
Shane T Kenny
Maciej Guzik
Branka Vasiljevic
Lidija Senerovic
Sanja Skaro
Jelena Radivojevic

Subjects

Microbiology

Topics
bioactive compounds cell proliferation pseudomonas aeruginosa candida albicans gram negative bacteria cell lines antimicrobial activity gram positive bacteria

Polyhydroxyalkanoate-based 3-hydroxyoctanoic acid and its derivatives as a platform of bioactive compounds. (2015)

Abstract A library of 18 different compounds was synthesized starting from (R)-3-hydroxyoctanoic acid which is derived from the bacterial polymer polyhydroxyalkanoate (PHA). Ten derivatives, including halo and unsaturated methyl and benzyl esters, were synthesized and characterized for the first time. Given that (R)-3-hydroxyalkanoic acids are known to have biological activity, the new compounds were evaluated for antimicrobial activity and in vitro antiproliferative effect with mammalian cell lines. The presence of the carboxylic group was essential for the antimicrobial activity, with minimal inhibitory concentrations against a panel of bacteria (Gram-positive and Gram-negative) and fungi (Candida albicans and Microsporum gypseum) in the range 2.8-7.0 mM and 0.1-6.3 mM, respectively. 3-Halogenated octanoic acids exhibited the ability to inhibit C. albicans hyphae formation. In addition, (R)-3-hydroxyoctanoic and (E)-oct-2-enoic acids inhibited quorum sensing-regulated pyocyanin production in the opportunistic pathogen Pseudomonas aeruginosa PAO1. Generally, derivatives did not inhibit mammalian cell proliferation even at 3-mM concentrations, while only (E)-oct-2-enoic and 3-oxooctanoic acid had IC50 values of 1.7 and 1.6 mM with the human lung fibroblast cell line.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Kevin E O'Connor, Jasmina Nikodinovic-Runic, Veselin Maslak, Shane T Kenny, Maciej Guzik, Branka Vasiljevic, Lidija Senerovic, Sanja Skaro, Jelena Radivojevic

Experts in our system

1
Kevin E O'Connor
University College Dublin
Total Publications: 52
 
2
Jasmina Nikodinovic-Runic
University College Dublin
Total Publications: 19
 
3
Shane T Kenny
University College Dublin
Total Publications: 15