Type

Other / n/a

Authors

Noel G McElvaney
Cedric Gunaratnam
Martin Clynes
Emmet O'Brien
Cormac McCarthy
Teck Boon Low
Michelle A Murray
Isabel Vega-Carrascal
Paula Meleady
Michael Henry
and 5 others

Subjects

Microbiology

Topics
enzyme linked immunosorbent assay alpha 1 antitrypsin proteomics disease progression antigens biomarkers neuronal young adult medicine and health sciences male neutrophils cell adhesion molecules adult humans cell adhesion molecules neuronal antigens cd fetal proteins cystic fibrosis female

A novel neutrophil derived inflammatory biomarker of pulmonary exacerbation in cystic fibrosis. (2012)

Abstract BACKGROUND: The focus of this study was to characterize a novel biomarker for cystic fibrosis (CF) that could reflect exacerbations of the disease and could be useful for therapeutic stratification of patients, or for testing of potential drug treatments. This study focused exclusively on a protein complex containing alpha-1 antitrypsin and CD16b (AAT:CD16b) which is released into the bloodstream from membranes of pro-inflammatory primed neutrophils.METHODS: Neutrophil membrane expression and extracellular levels of AAT and CD16b were quantified by flow cytometry, Western blot analysis and by 2D-PAGE. Interleukin-8 (IL-8), tumor necrosis factor-alpha (TNF-alpha) and AAT:CD16b complex were quantified in CF plasma (n=38), samples post antibiotic treatment for 14 days (n=10), chronic obstructive pulmonary disease (n=10), AAT deficient (n=10) and healthy control (n=14) plasma samples by ELISA.RESULTS: Cell priming with IL-8 and TNF-alpha caused release of the AAT:CD16b complex from the neutrophil cell membrane. Circulating plasma levels of IL-8, TNF-alpha and AAT:CD16b complex were significantly higher in patients with CF than in the other patient groups or healthy controls (PCONCLUSION: The results of this study have shown that levels of AAT:CD16b complex present in plasma correlate to the inflammatory status of patients. The AAT:CD16b biomarker may become a useful addition to the clinical diagnosis of exacerbations in CF.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
Ireland -> Royal College of Surgeons in Ireland -> RCSI Undergraduate Research
Ireland -> Royal College of Surgeons in Ireland -> Department of Medicine
Ireland -> Royal College of Surgeons in Ireland -> RCSI Undergraduate Research Articles

Full list of authors on original publication

Noel G McElvaney, Cedric Gunaratnam, Martin Clynes, Emmet O'Brien, Cormac McCarthy, Teck Boon Low, Michelle A Murray, Isabel Vega-Carrascal, Paula Meleady, Michael Henry and 5 others

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 190
 
2
Cedric Gunaratnam
Royal College of Surgeons in Ireland
Total Publications: 18
 
3
Martin Clynes
Dublin City University
Total Publications: 209
 
4
Cormac McCarthy
Royal College of Surgeons in Ireland
Total Publications: 19
 
5
Michelle A Murray
Royal College of Surgeons in Ireland
Total Publications: 8
 
6
Paula Meleady
Dublin City University
Total Publications: 95
 
7
Michael Henry
Dublin City University
Total Publications: 78