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Fergal O'Brien
Daniel J Kelly
Eric Farrell
Amos Matsiko
Emmet M Thompson



hypertrophy developmental engineering endochondral ossification anatomy bone mesenchymal stem cells bone regeneration intramembranous ossification

Recapitulating endochondral ossification: a promising route to in vivo bone regeneration. (2015)

Abstract Despite its natural healing potential, bone is unable to regenerate sufficient tissue within critical-sized defects, resulting in a non-union of bone ends. As a consequence, interventions are required to replace missing, damaged or diseased bone. Bone grafts have been widely employed for the repair of such critical-sized defects. However, the well-documented drawbacks associated with autografts, allografts and xenografts have motivated the development of alternative treatment options. Traditional tissue engineering strategies have typically attempted to direct in vitro bone-like matrix formation within scaffolds prior to implantation into bone defects, mimicking the embryological process of intramembranous ossification (IMO). Tissue-engineered constructs developed using this approach often fail once implanted, due to poor perfusion, leading to avascular necrosis and core degradation. As a result of such drawbacks, an alternative tissue engineering strategy, based on endochondral ossification (ECO), has begun to emerge, involving the use of in vitro tissue-engineered cartilage as a transient biomimetic template to facilitate bone formation within large defects. This is driven by the hypothesis that hypertrophic chondrocytes can secrete angiogenic and osteogenic factors, which play pivotal roles in both the vascularization of constructs in vivo and the deposition of a mineralized extracellular matrix, with resulting bone deposition. In this context, this review focuses on current strategies taken to recapitulate ECO, using a range of distinct cells, biomaterials and biochemical stimuli, in order to facilitate in vivo bone formation. Copyright © 2014 John Wiley & Sons, Ltd.
Collections Ireland -> Royal College of Surgeons in Ireland -> Anatomy Articles
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Full list of authors on original publication

Fergal O'Brien, Daniel J Kelly, Eric Farrell, Amos Matsiko, Emmet M Thompson

Experts in our system

Fergal J O'Brien
Royal College of Surgeons in Ireland
Total Publications: 268
Daniel Kelly
Trinity College Dublin
Total Publications: 190
Eric Farrell
Royal College of Surgeons in Ireland
Total Publications: 16
Amos Matsiko
Royal College of Surgeons in Ireland
Total Publications: 24
Emmet M Thompson
Royal College of Surgeons in Ireland
Total Publications: 17