Type

Journal Article

Authors

Paul McLoughlin
Michael P Keane
John Boylan
Aurelie Fabre
Sinead M Walsh
Simon C Rowan
Katherine U Gaynor
Noelle Murphy

Subjects

Biochemistry

Topics
human cells bone morphogenetic proteins epithelial cells bone growth 1 protein pulmonary fibrosis protein expression idiopathic pulmonary fibrosis

Altered Expression of Bone Morphogenetic Protein Accessory Proteins in Murine and Human Pulmonary Fibrosis. (2015)

Abstract Idiopathic pulmonary fibrosis is a chronic, progressive fibrotic disease with a poor prognosis. The balance between transforming growth factor β1 and bone morphogenetic protein (BMP) signaling plays an important role in tissue homeostasis, and alterations can result in pulmonary fibrosis. We hypothesized that multiple BMP accessory proteins may be responsible for maintaining this balance in the lung. Using the bleomycin mouse model for fibrosis, we examined an array of BMP accessory proteins for changes in mRNA expression. We report significant increases in mRNA expression of gremlin 1, noggin, follistatin, and follistatin-like 1 (Fstl1), and significant decreases in mRNA expression of chordin, kielin/chordin-like protein, nephroblastoma overexpressed gene, and BMP and activin membrane-bound inhibitor (BAMBI). Protein expression studies demonstrated increased levels of noggin, BAMBI, and FSTL1 in the lungs of bleomycin-treated mice and in the lungs of idiopathic pulmonary fibrosis patients. Furthermore, we demonstrated that transforming growth factor β stimulation resulted in increased expression of noggin, BAMBI, and FSTL1 in human small airway epithelial cells. These results provide the first evidence that multiple BMP accessory proteins are altered in fibrosis and may play a role in promoting fibrotic injury.
Collections Ireland -> University College Dublin -> PubMed

Full list of authors on original publication

Paul McLoughlin, Michael P Keane, John Boylan, Aurelie Fabre, Sinead M Walsh, Simon C Rowan, Katherine U Gaynor, Noelle Murphy

Experts in our system

1
Paul McLoughlin
University College Dublin
 
2
Michael P Keane
University College Dublin
Total Publications: 23
 
3
Aurélie Fabre
University College Dublin
Total Publications: 15
 
4
Simon C. Rowan
University College Dublin
Total Publications: 7