Type

Journal Article

Authors

Marco Ventura
Abelardo Margolles
Douwe van Sinderen
Miguel Gueimonde
Rebeca Martin
Borja Sanchez
Gabriele Andrea Lugli
Francesca Turroni
Sabrina Duranti
Elena Foroni
and 2 others

Subjects

Microbiology

Topics
sequence succession diversity bacterial phylogenetic microarray human intestinal tract disease communities health gut microbiota

Assessing the fecal microbiota: an optimized ion torrent 16S rRNA gene-based analysis protocol (2013)

Abstract Assessing the distribution of 16S rRNA gene sequences within a biological sample represents the current state-of-the-art for determination of human gut microbiota composition. Advances in dissecting the microbial biodiversity of this ecosystem have very much been dependent on the development of novel high-throughput DNA sequencing technologies, like the Ion Torrent. However, the precise representation of this bacterial community may be affected by the protocols used for DNA extraction as well as by the PCR primers employed in the amplification reaction. Here, we describe an optimized protocol for 16S rRNA gene-based profiling of the fecal microbiota.
Collections Ireland -> University College Cork -> APC Microbiome Institute
Ireland -> University College Cork -> College of Science, Engineering and Food Science
Ireland -> University College Cork -> APC Microbiome Institute- Journal Articles
Ireland -> University College Cork -> Microbiology
Ireland -> University College Cork -> Research Institutes and Centres
Ireland -> University College Cork -> Microbiology - Journal Articles

Full list of authors on original publication

Marco Ventura, Abelardo Margolles, Douwe van Sinderen, Miguel Gueimonde, Rebeca Martin, Borja Sanchez, Gabriele Andrea Lugli, Francesca Turroni, Sabrina Duranti, Elena Foroni and 2 others

Experts in our system

1
Marco Ventura
University College Cork
Total Publications: 31
 
2
Abelardo Margolles
University College Cork
 
3
Douwe van Sinderen
University College Cork
 
4
Miguel Gueimonde
Teagasc
Total Publications: 6
 
5
Borja Sanchez
Teagasc
 
6
Gabriele Andrea Lugli
University College Cork
Total Publications: 17
 
7
Francesca Turroni
University College Cork
Total Publications: 8
 
8
Sabrina Duranti
University College Cork