Type

Journal Article

Authors

David J Lynn
Alan G Fahey
Emma K Finlay
Francis Kearney
Donagh P. Berry
Daniel Bradley
Brian K Meredith

Subjects

Psychiatry

Topics
holstein friesian gwas single nucleotide polymorphisms genome wide association study mastitis association study genetic loci cell density snp qtl for scs and mastitis somatic cell score lupus erythematosus systemic

A genome-wide association study for somatic cell score using the Illumina high-density bovine beadchip identifies several novel QTL potentially related to mastitis susceptibility (2013)

Abstract Mastitis is an inflammation-driven disease of the bovine mammary gland that occurs in response to physical damage or infection and is one of the most costly production-related diseases in the dairy industry worldwide. We performed a genome-wide association study (GWAS) to identify genetic loci associated with somatic cell score (SCS), an indicator trait of mammary gland inflammation. A total of 702 Holstein-Friesian bulls were genotyped for 777,962 single nucleotide polymorphisms (SNPs) and associated with SCS phenotypes. The SCS phenotypes were expressed as daughter yield deviations (DYD) based on a large number of progeny performance records. A total of 138 SNPs on 15 different chromosomes reached genome-wide significance (corrected p-value ≤ 0.05) for association with SCS (after correction for multiple testing). We defined 28 distinct QTL regions and a number of candidate genes located in these QTL regions were identified. The most significant association (p-value = 1.70 × 10−7) was observed on chromosome 6. This QTL had no known genes annotated within it, however, the Ensembl Genome Browser predicted the presence of a small non-coding RNA (a Y RNA gene) in this genomic region. This Y RNA gene was 99% identical to human RNY4. Y RNAs are a rare type of non-coding RNA that were originally discovered due to their association with the autoimmune disease, systemic lupus erythematosus. Examining small-RNA sequencing (RNAseq) data being generated by us in multiple different mastitis-pathogen challenged cell-types has revealed that this Y RNA is expressed (but not differentially expressed) in these cells. Other QTL regions identified in this study also encoded strong candidate genes for mastitis susceptibility. A QTL region on chromosome 13, for example, was found to contain a cluster of β-defensin genes, a gene family with known roles in innate immunity. Due to the increased SNP density, this study also refined the boundaries for several known QTL for SCS and mastitis.
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Full list of authors on original publication

David J Lynn, Alan G Fahey, Emma K Finlay, Francis Kearney, Donagh P. Berry, Daniel Bradley, Brian K Meredith

Experts in our system

1
David J Lynn
Teagasc
Total Publications: 37
 
2
Alan G Fahey
University College Dublin
Total Publications: 34
 
3
Emma K Finlay
Teagasc
Total Publications: 11
 
4
Francis Kearney
Teagasc
Total Publications: 9
 
5
D P Berry
Teagasc
Total Publications: 243
 
6
Daniel Bradley
Trinity College Dublin
Total Publications: 84