Type

Other / n/a

Authors

Catherine M Greene
Stephen GJ Smith
Noel G McElvaney
Tidi Hassan
Sebastian Vencken

Subjects

Biochemistry

Topics
molecular sequence data oligonucleotides antisense antisense medicine and health sciences rna messenger rna cell line humans oligonucleotides messenger base sequence base pairing micrornas nucleic acid hybridization

miR-CATCH: microRNA capture affinity technology. (2015)

Abstract Several experimental methods exist to explore the microRNA (miRNA) regulome. These methods almost exclusively focus on multiple targets bound to a single, or perhaps a few miRNAs of interest. Here, we describe a microRNA capture affinity technology (miR-CATCH) which uses an affinity capture oligonucleotide to co-purify a single target messenger RNA (mRNA) together with all its endogenously bound miRNAs. This bench-top method is similar to RNA immunoprecipitation (RIP) and provides an experimental alternative to computational miRNA target prediction.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Medicine

Full list of authors on original publication

Catherine M Greene, Stephen GJ Smith, Noel G McElvaney, Tidi Hassan, Sebastian Vencken

Experts in our system

1
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
2
Stephen Smith
Trinity College Dublin
Total Publications: 27
 
3
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
4
Tidi Hassan
Royal College of Surgeons in Ireland
 
5
Sebastian Vencken
Royal College of Surgeons in Ireland
Total Publications: 13