Type

Other / n/a

Authors

Noel G McElvaney
Sanjay H Chotirmall
Cedric Gunaratnam
Catherine M Greene
Richard B Moss
Philip Murphy
Julie Renwick
Katie Dunne
Yael Gernez
Kevin Molloy
and 5 others

Subjects

Physiotherapy & Sport

Topics
medicine and health sciences aspergillus fumigatus allergic bronchopulmonary aspergillosis cd203c basophil activation test sensitization fev1 itraconazole body mass index cystic fibrosis flow cytometry

The basophil surface marker CD203c identifies Aspergillus species sensitization in patients with cystic fibrosis. (2016)

Abstract BACKGROUND: Colonization by Aspergillus fumigatus in patients with cystic fibrosis (CF) can cause A fumigatus sensitization and/or allergic bronchopulmonary aspergillosis (ABPA), which affects pulmonary function and clinical outcomes. Recent studies show that specific allergens upregulate the surface-expressed basophil marker CD203c in sensitized subjects, a response that can be readily measured by using flow cytometry.OBJECTIVE: We sought to identify A fumigatus sensitization in patients with CF by using the basophil activation test (BAT).METHODS: Patients with CF attending Beaumont Hospital were screened for study inclusion. BAT was used to identify A fumigatus sensitization. Serologic (total and A fumigatus-specific IgE), pulmonary function, and body mass index measurements were performed.RESULTS: The BAT discriminates A fumigatus-sensitized from nonsensitized patients with CF. Persistent isolation of A fumigatus in sputum is a significant risk factor for A fumigatus sensitization. Levels of the A fumigatus-stimulated basophil activation marker CD203c inversely correlated with pulmonary function and body mass index in A fumigatus-sensitized but not nonsensitized patients with CF. Total and A fumigatus-specific IgE, but not IgG, levels are increased in A fumigatus-sensitized patients with CF and ABPA when compared with those in A fumigatus-sensitized and nonsensitized patients with CF without ABPA. Itraconazole treatment did not affect A fumigatus sensitization.CONCLUSION: Combining the BAT with routine serologic testing allows classification of patients with CF into 3 groups: nonsensitized, A fumigatus-sensitized, and ABPA. Accurate and prompt identification of A fumigatus-associated clinical status might allow early and targeted therapeutic intervention, potentially improving clinical outcomes.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Medicine

Full list of authors on original publication

Noel G McElvaney, Sanjay H Chotirmall, Cedric Gunaratnam, Catherine M Greene, Richard B Moss, Philip Murphy, Julie Renwick, Katie Dunne, Yael Gernez, Kevin Molloy and 5 others

Experts in our system

1
Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
 
2
Sanjay H Chotirmall
Royal College of Surgeons in Ireland
Total Publications: 22
 
3
Cedric Gunaratnam
Royal College of Surgeons in Ireland
Total Publications: 18
 
4
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
 
5
Richard B Moss
Royal College of Surgeons in Ireland
Total Publications: 3
 
6
Philip Murphy
TU Dublin (Tallaght Campus)
Total Publications: 30
 
7
Julie Renwick
TU Dublin (Tallaght Campus)
Total Publications: 10
 
8
Katie Dunne
Trinity College Dublin
Total Publications: 10
 
9
Yael Gernez
Royal College of Surgeons in Ireland
Total Publications: 3
 
10
Kevin Molloy
Royal College of Surgeons in Ireland
Total Publications: 19