Other / n/a


Noel G McElvaney
Catherine M Greene
Rosaleen Devery
Marianne S Muhlebach
Michael M Tunney
Matthew Wolfgang
Joe Hatch
Paul McNally
Dubhfeasa Slattery
Fiona Healy
and 6 others



gas western epithelial cells fatty acids polymerase chain reaction infant adult chromatography gas preschool humans cystic fibrosis transmembrane conductance regulator cystic fibrosis adolescent age factors chromatography respiratory mucosa anaerobic female bronchoalveolar lavage fluid enzyme linked immunosorbent assay up regulation medicine and health sciences child preschool male bacteria anaerobic young adult blotting blotting western child middle aged bacteria

The Role of Short-Chain Fatty Acids, Produced by Anaerobic Bacteria, in the Cystic Fibrosis Airway. (2015)

Abstract RATIONALE: Anaerobic bacteria are present in large numbers in the airways of people with cystic fibrosis (PWCF). In the gut, anaerobes produce short-chain fatty acids (SCFAs) that modulate immune and inflammatory processes.OBJECTIVES: To investigate the capacity of anaerobes to contribute to cystic fibrosis (CF) airway pathogenesis via SCFAs.METHODS: Samples of 109 PWCF were processed using anaerobic microbiological culture with bacteria present identified by 16S RNA sequencing. SCFA levels in anaerobic supernatants and bronchoalveolar lavage (BAL) were determined by gas chromatography. The mRNA and/or protein expression of two SCFA receptors, GPR41 and GPR43, in CF and non-CF bronchial brushings and 16HBE14o(-) and CFBE41o(-) cells were evaluated using reverse transcription polymerase chain reaction, Western blot analysis, laser scanning cytometry, and confocal microscopy. SCFA-induced IL-8 secretion was monitored by ELISA.MEASUREMENTS AND MAIN RESULTS: Fifty-seven (52.3%) of 109 PWCF were anaerobe positive. Prevalence increased with age, from 33.3% to 57.7% in PWCF younger (n = 24) and older (n = 85) than 6 years of age. All evaluated anaerobes produced millimolar concentrations of SCFAs, including acetic, propionic, and butyric acids. SCFA levels were higher in BAL samples of adults than in those of children. GPR41 levels were elevated in CFBE41o(-) versus 16HBE14o(-) cells; CF versus non-CF bronchial brushings; and 16HBE14o(-) cells after treatment with cystic fibrosis transmembrane conductance regulator inhibitor CFTR(inh)-172, CF BAL, or inducers of endoplasmic reticulum stress. SCFAs induced a dose-dependent and pertussis toxin-sensitive IL-8 response in bronchial epithelial cells, with a higher production of IL-8 in CFBE41o(-) than in 16HBE14o(-) cells.CONCLUSIONS: This study illustrates that SCFAs contribute to excessive production of IL-8 in CF airways colonized with anaerobes via up-regulated GPR41.
Collections Ireland -> Royal College of Surgeons in Ireland -> Medicine Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Medicine

Full list of authors on original publication

Noel G McElvaney, Catherine M Greene, Rosaleen Devery, Marianne S Muhlebach, Michael M Tunney, Matthew Wolfgang, Joe Hatch, Paul McNally, Dubhfeasa Slattery, Fiona Healy and 6 others

Experts in our system

Noel G McElvaney
Royal College of Surgeons in Ireland
Total Publications: 194
Catherine M Greene
Royal College of Surgeons in Ireland
Total Publications: 150
R Devery
Dublin City University
Total Publications: 36
Paul McNally
Royal College of Surgeons in Ireland
Total Publications: 26