Type

Other / n/a

Authors

Kate L Lambertsen
Jochen HM Prehn
Shona Pfeiffer
Tomas Deierborg
Martina Svensson
Hans G König
Gang Chen
Beatrice D'Orsi
Maria L Elkjær
Helena Bonner
and 1 others

Subjects

Psychiatry

Topics
physiology physics inflammation organotypic hippocampal slice cultures focal cerebral ischemia central nervous system bid neuronal injury

BID Mediates Oxygen-Glucose Deprivation-Induced Neuronal Injury in Organotypic Hippocampal Slice Cultures and Modulates Tissue Inflammation in a Transient Focal Cerebral Ischemia Model without Changing Lesion Volume. (2016)

Abstract The BH3 interacting-domain death agonist (BID) is a pro-apoptotic protein involved in death receptor-induced and mitochondria-mediated apoptosis. Recently, it has also been suggested that BID is involved in the regulation of inflammatory responses in the central nervous system. We found that BID deficiency protected organotypic hippocampal slice cultures in vitro from neuronal injury induced by oxygen-glucose deprivation. In vivo, BID-knockout (KO) mice and wild type (WT) mice were subjected to 60 min of transient middle cerebral artery occlusion (tMCAO) to induce focal cerebral ischemia, and allowed to recover for 24 h. Infarct volumes and functional outcome were assessed and the inflammatory response was evaluated using immunofluorescence, Western blotting, quantitative PCR (qPCR) and Mesoscale multiplex analysis. We observed no difference in the infarct volume or neurological outcome between BID-KO and WT mice. The inflammatory response was reduced by BID deficiency as indicated by a change in microglial/leukocyte response. In conclusion, our data suggest that BID deficiency is neuroprotective in an in vitro model and modulates the inflammatory response to focal cerebral ischemia in vivo. However, this is not translated into a robust neuroprotection in vivo.
Collections Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

Kate L Lambertsen, Jochen HM Prehn, Shona Pfeiffer, Tomas Deierborg, Martina Svensson, Hans G König, Gang Chen, Beatrice D'Orsi, Maria L Elkjær, Helena Bonner and 1 others

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Shona Pfeiffer
Royal College of Surgeons in Ireland
Total Publications: 14
 
3
Hans Georg König
Royal College of Surgeons in Ireland
Total Publications: 22
 
4
G Chen
Royal College of Surgeons in Ireland
Total Publications: 19
 
5
Beatrice D'Orsi
Royal College of Surgeons in Ireland
Total Publications: 14
 
6
Helena P Bonner
Royal College of Surgeons in Ireland
Total Publications: 15