Type

Other / n/a

Authors

Jochen HM Prehn
Annette T Byrne
John J Callanan
Donncha F O'Brien
Patrick Dicker
Monika A Jarzabek
Kieron J Sweeney

Subjects

Medicine & Nursing

Topics
cell line tumor reproducibility of results luminescent measurements physics tumor animals glioblastoma random allocation neurosurgical procedures cell line follow up studies neoplasm transplantation local humans physiology brain neoplasms kaplan meier estimate neoplasm recurrence local neoplasm recurrence optical imaging rats

Validation of an imageable surgical resection animal model of Glioblastoma (GBM). (2014)

Abstract BACKGROUND: Glioblastoma (GBM) is the most common and malignant primary brain tumour having a median survival of just 12-18 months following standard therapy protocols. Local recurrence, post-resection and adjuvant therapy occurs in most cases.NEW METHOD: U87MG-luc2-bearing GBM xenografts underwent 4.5mm craniectomy and tumour resection using microsurgical techniques. The cranial defect was repaired using a novel modified cranial window technique consisting of a circular microscope coverslip held in place with glue.RESULTS: Immediate post-operative bioluminescence imaging (BLI) revealed a gross total resection rate of 75%. At censor point 4 weeks post-resection, Kaplan-Meier survival analysis revealed 100% survival in the surgical group compared to 0% in the non-surgical cohort (p=0.01). No neurological defects or infections in the surgical group were observed. GBM recurrence was reliably imaged using facile non-invasive optical bioluminescence (BLI) imaging with recurrence observed at week 4.COMPARISON WITH EXISTING METHOD(S): For the first time, we have used a novel cranial defect repair method to extend and improve intracranial surgical resection methods for application in translational GBM rodent disease models. Combining BLI and the cranial window technique described herein facilitates non-invasive serial imaging follow-up.CONCLUSION: Within the current context we have developed a robust methodology for establishing a clinically relevant imageable GBM surgical resection model that appropriately mimics GBM recurrence post resection in patients.
Collections Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

Jochen HM Prehn, Annette T Byrne, John J Callanan, Donncha F O'Brien, Patrick Dicker, Monika A Jarzabek, Kieron J Sweeney

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Annette T Byrne
Royal College of Surgeons in Ireland
Total Publications: 41
 
3
John J Callanan
University College Dublin
Total Publications: 60
 
4
Donncha F O'Brien
Royal College of Surgeons in Ireland
Total Publications: 19
 
5
Patrick Dicker
Royal College of Surgeons in Ireland
Total Publications: 95
 
6
Monika A Jarzabek
Royal College of Surgeons in Ireland
Total Publications: 12