Type

Other / n/a

Authors

Jochen HM Prehn
Deborah A McNamara
Elaine W Kay
Caoimhin G Concannon
Orna Bacon
Niamh McCawley
Suzanne Hector
Joanna Fay
Andreas U Lindner
Áine C Murphy
and 2 others

Subjects

Biochemistry

Topics
upr unfolded protein response calnexin colorectal cancer er stress physiology grp78 physics grp94

Calnexin, an ER-induced protein, is a prognostic marker and potential therapeutic target in colorectal cancer. (2016)

Abstract BACKGROUND: Colorectal cancer (CRC) is a leading cause of cancer mortality in the Western world and commonly treated with genotoxic chemotherapy. Stress in the endoplasmic reticulum (ER) was implicated to contribute to chemotherapeutic resistance. Hence, ER stress related protein may be of prognostic or therapeutic significance.METHODS: The expression levels of ER stress proteins calnexin, calreticulin, GRP78 and GRP94 were determined in n = 23 Stage II and III colon cancer fresh frozen tumour and matched normal tissue samples. Data were validated in a cohort of n = 11 rectal cancer patients treated with radiochemotherapy in the neoadjuvant setting. The calnexin gene was silenced using siRNA in HCT116 cells.RESULTS: There were no increased levels of ER stress proteins in tumour compared to matched normal tissue samples in Stage II or III CRC. However, increased calnexin protein levels were predictive of poor clinical outcome in the patient cohort. Data were validated in the rectal cancer cohort treated in the neoadjuvant setting. Calnexin gene-silencing significantly reduced cell survival and increased cancer cell susceptibility to 5FU chemotherapy.CONCLUSION: Increased tumour protein levels of calnexin may be of prognostic significance in CRC, and calnexin may represent a potential target for future therapies.
Collections Ireland -> Royal College of Surgeons in Ireland -> Physiology and Medical Physics Articles
Ireland -> Royal College of Surgeons in Ireland -> Department of Physiology and Medical Physics

Full list of authors on original publication

Jochen HM Prehn, Deborah A McNamara, Elaine W Kay, Caoimhin G Concannon, Orna Bacon, Niamh McCawley, Suzanne Hector, Joanna Fay, Andreas U Lindner, Áine C Murphy and 2 others

Experts in our system

1
Jochen H M Prehn
Royal College of Surgeons in Ireland
Total Publications: 206
 
2
Deborah A McNamara
Royal College of Surgeons in Ireland
Total Publications: 17
 
3
Elaine W Kay
Royal College of Surgeons in Ireland
Total Publications: 157
 
4
Caoimhín G Concannon
Royal College of Surgeons in Ireland
Total Publications: 46
 
5
Orna Bacon
Royal College of Surgeons in Ireland
Total Publications: 13
 
6
Niamh McCawley
Royal College of Surgeons in Ireland
Total Publications: 9
 
7
Suzanne Hector
Royal College of Surgeons in Ireland
Total Publications: 13
 
8
Joanna Fay
Royal College of Surgeons in Ireland
Total Publications: 24
 
9
Andreas U Lindner
Royal College of Surgeons in Ireland
Total Publications: 13
 
10
Áine C Murphy
Royal College of Surgeons in Ireland
Total Publications: 8